Probable DNA repair helicase ercc3 (ercc3) Recombinant Protein | ercc3 recombinant protein

Recombinant Schizosaccharomyces pombe Probable DNA repair helicase ercc3 (ercc3), partial

• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Probable DNA repair helicase ercc3 (ercc3); Recombinant Schizosaccharomyces pombe Probable DNA repair helicase ercc3 (ercc3); partial; ercc3 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Length: 804

• Species: Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 19114386
• NCBI GeneID: 2542174
• NCBI Accession #: NP_593474.1
• NCBI GenBank Nucleotide #: NM_001018907.2
• UniProt Accession #: O13768; Q9US81
• Molecular Weight: 91,345 Da
• NCBI Official Full Name: transcription factor TFIIH complex ERCC-3 subunit
• NCBI Official Symbol: ptr8
• NCBI Protein Information: transcription factor TFIIH complex ERCC-3 subunit
• UniProt Protein Name: General transcription and DNA repair factor IIH helicase subunit XPB
• Protein Family: Probable DNA repair helicase
• UniProt Gene Name: ptr8
• UniProt Synonym Gene Names: TFIIH subunit XPB; TFB subunit ercc3

Uniprot Description

ATP-dependent 3'-5' DNA helicase, component of the general transcription and DNA repair factor IIH (TFIIH) core complex, which is involved in general and transcription-coupled nucleotide excision repair (NER) of damaged DNA and, when complexed to TFIIK, in RNA transcription by RNA polymerase II. In NER, TFIIH acts by opening DNA around the lesion to allow the excision of the damaged oligonucleotide and its replacement by a new DNA fragment. The ATPase activity of XPB/ptr8, but not its helicase activity, is required for DNA opening. In transcription, TFIIH has an essential role in transcription initiation. When the pre-initiation complex (PIC) has been established, TFIIH is required for promoter opening and promoter escape. The ATP-dependent helicase activity of XPB/ptr8 is required for promoter opening and promoter escape. Phosphorylation of the C-terminal tail (CTD) of the largest subunit of RNA polymerase II by the kinase module TFIIK controls the initiation of transcription (). Plays a role in mRNA export (PubMed:17212653).