Pleiotrophin Recombinant Protein | PTN recombinant protein
Pleiotrophin, Recombinant, Human (PTN, HARP, Heparin Affin Regulatory Peptide)
Gene Names
PTN; HARP; HBNF; HBGF8; NEGF1
Purity
Highly Purified95% (SDS-PAGE); Three-step procedure using affinity Ni-NTA chromatography and size exclusion chromatography before and after refolding.
Synonyms
Pleiotrophin; Recombinant; Human (PTN; HARP; Heparin Affin Regulatory Peptide); PTN recombinant protein
Host
E Coli
Specificity
The amino acid sequence of the recombinant human OSF-1 is 100% homologous to the amino acid sequence of the human OSF-1 without signal sequence.
Purity/Purification
Highly Purified
95% (SDS-PAGE); Three-step procedure using affinity Ni-NTA chromatography and size exclusion chromatography before and after refolding.
95% (SDS-PAGE); Three-step procedure using affinity Ni-NTA chromatography and size exclusion chromatography before and after refolding.
Form/Format
Supplied as a lyophilized powder in 0.1M phosphate buffer, 0.1M sodium chloride, pH 7.2.
Sequence
MKHHHHHHHM LVPRGSGKKE KPEKKVKKSD CGEWQWSVCV PTSGDCGLGT REGTRTGAEC KQTMKTQRCK IPCNWKKQFG AECKYQFQAW GECDLNTALK TRTGSLKRAL HNAECQKTVT ISKPCGKLTK PKPQAESKKK KKEGKKQEKM LD
Application Notes
Western blotting, ELISA
Solubility
Add 0.2 ml of PBS pH 7.2 and let the lyophilized pellet dissolve completely. Storage: Store lyophilized protein at -20 degree C. Aliquot the product after reconstitution to avoid repeated freezing/thawing cycles. Reconstituted protein can be stored at 4 degree C for a limited period of time; it does not show any change after two weeks at 4 degree C.
Preparation and Storage
-20 degree C
Stability: The lyophilized protein remains stable until the expiry date when stored at -20 degree C.
Stability: The lyophilized protein remains stable until the expiry date when stored at -20 degree C.
Related Product Information for PTN recombinant protein
Pleiotrophin (Osteoblast-Specific Factor-1, OSF-1) contains 136 amino acid residues. The sequence is very rich in cationic amino acids (24% of the residues); lysine cluster sequences are found in the Nterminal and C-terminal ends of the structure. The OSF-1 gene was shown by Northern blotting analysis to be expressed in mouse calvarial osteoblast-enriched cells and in mouse brain tissues, but not in thymus, spleen, kidney, liver, lung, testis or heart. Pliotrophin has the ability to promote adhesion, migration, expansion, and differentiation of human osteoprogenitor cells. In addition to certain types of cancer, the embryonic growth and differentiation factor pleiotrophin is found also in adults in inflammatory diseases. In osteoarthritis, pleiotrophin is especially expressed in early stages, and its concentrations in the synovial fluid could serve as a marker for the progress of the disease. Pleitrophin might be involved in cartilage repair in osteoarthritis, in particular, in earlier stages.
Recombinant Human Pleiotrophin contains His-Tagged Fusion Protein, produced in E. coli, its molecular weight is 17.3kD protein containing 136 amino acid residues of the human OSF-1 and 16 additional amino acid residues-HisTag, thrombin cleavage site.
Recombinant Human Pleiotrophin contains His-Tagged Fusion Protein, produced in E. coli, its molecular weight is 17.3kD protein containing 136 amino acid residues of the human OSF-1 and 16 additional amino acid residues-HisTag, thrombin cleavage site.
Product Categories/Family for PTN recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
17.3kD
NCBI Official Full Name
pleiotrophin
NCBI Official Synonym Full Names
pleiotrophin
NCBI Official Symbol
PTN
NCBI Official Synonym Symbols
HARP; HBNF; HBGF8; NEGF1
NCBI Protein Information
pleiotrophin; HBBM; OSF-1; HB-GAM; HBGF-8; HBNF-1; OTTHUMP00000208521; OTTHUMP00000208522; osteoblast-specific factor 1; heparin-binding brain mitogen; heparin binding growth factor 8; heparin-binding growth factor 8; heparin affin regulatory protein; neurite growth-promoting factor 1; heparin-binding growth-associated molecule; heparin-binding neurite outgrowth-promoting factor 1; pleiotrophin (heparin binding growth factor 8, neurite growth-promoting factor 1)
UniProt Protein Name
Pleiotrophin
Protein Family
UniProt Gene Name
PTN
UniProt Synonym Gene Names
HBNF1; NEGF1
UniProt Entry Name
PTN_HUMAN
Uniprot Description
Function: Heparin binding mitogenic protein. Has neurite extension activity. Ref.3
Subcellular location: Secreted.
Tissue specificity: Osteoblast and brain.
Induction: By heparin and retinoic acid. Ref.3 Ref.15
Sequence similarities: Belongs to the pleiotrophin family.
Research Articles on PTN
Similar Products
Product Notes
The PTN ptn (Catalog #AAA650703) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. Western blotting, ELISA. Researchers should empirically determine the suitability of the PTN ptn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MKHHHHHHHM LVPRGSGKKE KPEKKVKKSD CGEWQWSVCV PTSGDCGLGT REGTRTGAEC KQTMKTQRCK IPCNWKKQFG AECKYQFQAW GECDLNTALK TRTGSLKRAL HNAECQKTVT ISKPCGKLTK PKPQAESKKK KKEGKKQEKM LD. It is sometimes possible for the material contained within the vial of "Pleiotrophin, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
