SDS-Page
(Recombinant FAK protein gel 5% SDS-PAGE gel, stained with Coomassie Blue MW: 120.5 kDa Purity: >65% )
FAK recombinant protein
Recombinant FAK protein
Shipping Temp: Dry Ice
SDS-Page
(Recombinant FAK protein gel 5% SDS-PAGE gel, stained with Coomassie Blue MW: 120.5 kDa Purity: >65% )
SDS-Page
(Recombinant FAK protein gel 5% SDS-PAGE gel, stained with Coomassie Blue MW: 120.5 kDa Purity: >65% )
Testing Data
(HTRF assay for FAK protein activity 1 uM TK substrate was incubated with increasing concentrations of FAK protein and 100 uM ATP in reaction buffer for 1 hour. The detection reagents were added and incubated with the reactions for 1 hr. TK antibody was used to detect the products. All operations and reactions were performed at room temperature, and HTRF KinEASE TK assay was used to detect the enzymatic activity. )
Testing Data
(HTRF assay for FAK protein activity 1 uM TK substrate was incubated with increasing concentrations of FAK protein and 100 uM ATP in reaction buffer for 1 hour. The detection reagents were added and incubated with the reactions for 1 hr. TK antibody was used to detect the products. All operations and reactions were performed at room temperature, and HTRF KinEASE TK assay was used to detect the enzymatic activity. )
Testing Data
(HTRF assay for FAK protein activity 1 uM TK substrate was incubated with increasing concentrations of FAK protein and 100 uM ATP in reaction buffer for 1 hour. The detection reagents were added and incubated with the reactions for 1 hr. TK antibody was used to detect the products. All operations and reactions were performed at room temperature, and HTRF KinEASE TK assay was used to detect the enzymatic activity.)
Testing Data
(HTRF assay for FAK protein activity 1 uM TK substrate was incubated with increasing concentrations of FAK protein and 100 uM ATP in reaction buffer for 1 hour. The detection reagents were added and incubated with the reactions for 1 hr. TK antibody was used to detect the products. All operations and reactions were performed at room temperature, and HTRF KinEASE TK assay was used to detect the enzymatic activity.)
Background: FAK (Focal Adhesion Kinase 1), also known as PTK2 (Protein Tyrosine Kinase 2), is a non-receptor protein-tyrosine kinase that plays an essential role in regulating cell migration, adhesion, spreading, reorganization of the actin cytoskeleton, formation and disassembly of focal adhesions and cell protrusions, cell cycle progression, cell proliferation and apoptosis. It is required for early embryonic development and placenta development, embryonic angiogenesis, normal cardiomyocyte migration and proliferation and normal heart development. FAK regulates axon growth and neuronal cell migration, axon branching and synapse formation, and it is required for normal development of the nervous system. It also plays a role in osteogenesis and differentiation of osteoblasts. FAK can function in integrin signal transduction, but also in signaling downstream of numerous growth factor receptors, G-protein coupled receptors (GPCR), EPHA2, netrin receptors and LDL receptors. It can form multisubunit signaling complexes with SRC and SRC family members upon activation; this leads to the phosphorylation of additional tyrosine residues, creating binding sites for scaffold proteins, effectors and substrates. FAK also can regulate numerous signaling pathways, promote activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascade, MAPK1/ERK2, MAPK3/ERK1 and the MAP kinase signaling cascade. It can recruit the ubiquitin ligase MDM2 to P53/TP53 in the nucleus, and thereby regulates P53/TP53 activity, P53/TP53 ubiquitination and proteasomal degradation.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a cytoplasmic protein tyrosine kinase which is found concentrated in the focal adhesions that form between cells growing in the presence of extracellular matrix constituents. The encoded protein is a member of the FAK subfamily of protein tyrosine kinases but lacks significant sequence similarity to kinases from other subfamilies. Activation of this gene may be an important early step in cell growth and intracellular signal transduction pathways triggered in response to certain neural peptides or to cell interactions with the extracellular matrix. Several transcript variants encoding different isoforms have been found for this gene, but the full-length natures of only four of them have been determined. [provided by RefSeq, Oct 2015]
Uniprot Description
FAK: a tyrosine kinase of the FAK family required for cell migration and contact-dependent survival signaling. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Downstream of integrins and Src, upstream of Ras/MAPK. Localizes to focal adhesions that form between cells growing in the presence of extracellular matrix constituents. Interacts with CAS family members and with GIT1, SORBS1 and BCAR3. Interacts with Shb. Required for full Ras transformation of fibroblasts. Increased expression in breast and other cancers, related to chromosome 8q amplification. Overexpression and activation associated with increased migration, invasion and progression of ovarian cancer, and with progression in hepatocellular carcinoma, thyroid cancer, and acute myelogenous leukemia. siRNA increases chemosensitivity of pancreatic adenocarcinoma xenografts. Inhibitor: ISI15421 (antisense). Four splice-variant isoforms have been observed.
Protein type: Protein kinase, TK; Kinase, protein; EC 2.7.10.2; Protein kinase, tyrosine (non-receptor); TK group; Fak family
Chromosomal Location of Human Ortholog: 8q24.3
Cellular Component: extrinsic to internal side of plasma membrane; focal adhesion; cytoskeleton; lamellipodium; cytoplasm; apical plasma membrane; stress fiber; plasma membrane; microtubule organizing center; cell cortex; cytosol; nucleus
Molecular Function: JUN kinase binding; protein binding; signal transducer activity; protein-tyrosine kinase activity; non-membrane spanning protein tyrosine kinase activity; SH2 domain binding; actin binding; protein kinase binding; ATP binding; receptor binding; protein kinase activity
Biological Process: heart morphogenesis; axon guidance; extracellular matrix organization and biogenesis; establishment of nucleus localization; peptidyl-tyrosine phosphorylation; apoptosis; protein amino acid autophosphorylation; cell motility involved in cell locomotion; neuron migration; negative regulation of synaptogenesis; regulation of cell shape; regulation of cell adhesion mediated by integrin; transforming growth factor beta receptor signaling pathway; positive regulation of cell proliferation; ephrin receptor signaling pathway; negative regulation of axonogenesis; angiogenesis; vasculogenesis; cell structure disassembly during apoptosis; placenta development; integrin-mediated signaling pathway; epidermal growth factor receptor signaling pathway; platelet activation; regulation of osteoblast differentiation; central nervous system neuron axonogenesis; signal complex assembly; positive regulation of phosphoinositide 3-kinase activity; cytoskeleton organization and biogenesis; microtubule cytoskeleton organization and biogenesis; negative regulation of organ growth; regulation of cell proliferation; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; embryonic development; establishment of cell polarity; positive regulation of protein kinase activity; regulation of focal adhesion formation; endothelial cell migration; innate immune response; positive regulation of protein amino acid phosphorylation; negative regulation of cell-cell adhesion; blood coagulation; vascular endothelial growth factor receptor signaling pathway; positive regulation of cell migration; regulation of cytoskeleton organization and biogenesis; negative regulation of apoptosis
Research Articles on FAK
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Product Notes
The FAK ptk2 (Catalog #AAA389165) is a Recombinant Protein produced from Baculovirus and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "FAK, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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