Human Parathyroid hormone-related protein ELISA Kit | PTHLH elisa kit

Human Parathyroid hormone-related protein ELISA Kit

Cat. Num. AAA760563

• Gene Names: PTHLH; HHM; PLP; BDE2; PTHR; PTHRP
• Reactivity: Human
• Synonyms: Parathyroid hormone-related protein; Human Parathyroid hormone-related protein ELISA Kit; PTHrP (Parathyroid Hormone Related Protein)/PTHLH/BDE2/HHM/Osteostatin/PTHRP/parathyroid hormone-like hormone/Parathyroid hormone-like protein/parathyroid hormone-related protein/PLPosteostatin/PTHR/PTH-related protein/PTH-rP/parathyroid hormone-like related protein; PTHLH elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of PTHLH. No significant cross-reactivity or interference between PTHLH and analogues was observed.
• Sequence Length: 177

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 15.6-1000pg/ml
• Sensitivity: <9.375pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level PTHLH were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level PTHLH were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for PTHLH elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- PTHLH antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- PTHLH antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the PTHLH amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of PTHLH can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 190726
• NCBI GeneID: 5744
• Molecular Weight: 23,942 Da
• NCBI Official Full Name: parathyroid hormone-related protein
• NCBI Official Synonym Full Names: parathyroid hormone-like hormone
• NCBI Official Symbol: PTHLH
• NCBI Official Synonym Symbols: HHM; PLP; BDE2; PTHR; PTHRP
• NCBI Protein Information: parathyroid hormone-related protein
• UniProt Protein Name: Parathyroid hormone-related protein
• Protein Family: Parathyroid hormone-related protein
• UniProt Gene Name: PTHLH
• UniProt Synonym Gene Names: PTHRP; PTH-rP; PTHrP; PLP
• UniProt Entry Name: PTHR_HUMAN

NCBI Description

The protein encoded by this gene is a member of the parathyroid hormone family. This hormone, via its receptor, PTHR1, regulates endochondral bone development and epithelial-mesenchymal interactions during the formation of the mammary glands and teeth. It is responsible for most cases of humoral hypercalcemia of malignancy, and mutations in this gene are associated with brachydactyly type E2 (BDE2). Alternatively spliced transcript variants have been found for this gene. There is also evidence for alternative translation initiation from non-AUG (CUG and GUG) start sites, downstream of the initiator AUG codon, resulting in nuclear forms of this hormone. [provided by RefSeq, Nov 2013]

Uniprot Description

PTHrP: a member of the parathyroid hormone family. This hormone regulates endochondral bone development and epithelial-mesenchymal interactions during the formation of the mammary glands and teeth. This hormone is involved in lactation possibly by regulating the mobilization and transfer of calcium to the milk. The receptor of this hormone, PTHR1, is responsible for most cases of humoral hypercalcemia of malignancy. Three alternatively spliced isoforms have been described.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 12p12.1-p11.2

Cellular Component: cytoplasm; extracellular region; extracellular space; Golgi apparatus; nucleoplasm

Molecular Function: hormone activity; peptide hormone receptor binding

Biological Process: alveolus development; cAMP metabolic process; cell-cell signaling; endochondral ossification; endoderm development; epidermis development; epithelial cell differentiation; female pregnancy; G-protein signaling, adenylate cyclase activating pathway; negative regulation of cell proliferation; negative regulation of chondrocyte differentiation; negative regulation of transcription factor activity; osteoblast development; positive regulation of cAMP biosynthetic process; positive regulation of cell proliferation; protein processing; regulation of gene expression; skeletal development; surfactant homeostasis

Disease: Brachydactyly, Type E2