SDS-Page
PTGR2 recombinant protein
PTGR2, 1-351aa, Human, His tag, E Coli
Gene Names
PTGR2; PGR2; ZADH1; HEL-S-298
Applications
SDS-Page
Purity
> 90% by SDS-PAGE
Synonyms
PTGR2; 1-351aa; Human; His tag; E Coli; Prostaglandin reductase 2; PGR2; ZADH1; PTGR2 recombinant protein
Host
E Coli
Purity/Purification
> 90% by SDS-PAGE
Form/Format
Liquid. In 20 mM Tris-HCl Buffer, pH8.0, 10% glycerol, 1mM DTT, 50mM NaCl
Concentration
1mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMIVQRV VLNSRPGKNG NPVAENFRME EVYLPDNINE GQVQVRTLYL SVDPYMRCRM NEDTGTDYIT PWQLSQVVDG GGIGIIEESK HTNLTKGDFV TSFYWPWQTK VILDGNSLEK VDPQLVDGHL SYFLGAIGMP GLTSLIGIQE KGHITAGSNK TMVVSGAAGA CGSVAGQIGH FLGCSRVVGI CGTHEKCILL TSELGFDAAI NYKKDNVAEQ LRESCPAGVD VYFDNVGGNI SDTVISQMNE NSHIILCGQI SQYNKDVPYP PPLSPAIEAI QKERNITRER FLVLNYKDKF EPGILQLSQW FKEGKLKIKE TVINGLENMG AAFQSMMTGG NIGKQIVCIS EEISL
Sequence Length
1-351
Applicable Applications for PTGR2 recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 2-8°C for 1 week. For long term storage, aliquot and store at -20°C to -80°C. Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for PTGR2 recombinant protein
PTGR2 belongs to the medium-chain dehydrogenase/reductase superfamily. PTGR2 catalyzes an NADPH-dependent reduction of the conjugated alpha,beta-unsaturated double bond of 15-keto-PGE (2), a key step in terminal inactivation of prostaglandins and suppression of PPARgamma-mediated adipocyte differentiation. Selective inhibition of PTGR2 may contribute to the improvement of insulin sensitivity with fewer side effects. Recombinant human PTGR2 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for PTGR2 recombinant protein
References
Wu Y.H., et al. (2008) Structure 16:1714-1723; Auld, D.S. et al. (2008) Cell. Mol. Life Sci. 65: 3961-3970.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
41.1 kDa (375aa), confirmed by MALDI-TOF
NCBI Official Full Name
prostaglandin reductase 2
NCBI Official Synonym Full Names
prostaglandin reductase 2
NCBI Official Symbol
PTGR2
NCBI Official Synonym Symbols
PGR2; ZADH1; HEL-S-298
NCBI Protein Information
prostaglandin reductase 2
UniProt Protein Name
Prostaglandin reductase 2
Protein Family
UniProt Gene Name
PTGR2
UniProt Synonym Gene Names
ZADH1; PRG-2
NCBI Description
This gene encodes an enzyme involved in the metabolism of prostaglandins. The encoded protein catalyzes the NADPH-dependent conversion of 15-keto-prostaglandin E2 to 15-keto-13,14-dihydro-prostaglandin E2. This protein may also be involved in regulating activation of the peroxisome proliferator-activated receptor. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2009]
Uniprot Description
Functions as 15-oxo-prostaglandin 13-reductase and acts on 15-keto-PGE1, 15-keto-PGE2, 15-keto-PGE1-alpha and 15-keto-PGE2-alpha with highest activity towards 15-keto-PGE2. Overexpression represses transcriptional activity of PPARG and inhibits adipocyte differentiation ().
Research Articles on PTGR2
Similar Products
Product Notes
The PTGR2 ptgr2 (Catalog #AAA204775) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's PTGR2 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the PTGR2 ptgr2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGSHMIVQRV VLNSRPGKNG NPVAENFRME EVYLPDNINE GQVQVRTLYL SVDPYMRCRM NEDTGTDYIT PWQLSQVVDG GGIGIIEESK HTNLTKGDFV TSFYWPWQTK VILDGNSLEK VDPQLVDGHL SYFLGAIGMP GLTSLIGIQE KGHITAGSNK TMVVSGAAGA CGSVAGQIGH FLGCSRVVGI CGTHEKCILL TSELGFDAAI NYKKDNVAEQ LRESCPAGVD VYFDNVGGNI SDTVISQMNE NSHIILCGQI SQYNKDVPYP PPLSPAIEAI QKERNITRER FLVLNYKDKF EPGILQLSQW FKEGKLKIKE TVINGLENMG AAFQSMMTGG NIGKQIVCIS EEISL. It is sometimes possible for the material contained within the vial of "PTGR2, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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