Proteasome Subunit Beta Type 5 Recombinant Protein | PSMB5 recombinant protein

Recombinant Human Proteasome Subunit Beta Type 5

Cat. Num. AAA144287

• Gene Names: PSMB5; X; MB1; LMPX
• Purity: Greater than 90.0% as determined by SDS-PAGE.
• Synonyms: Proteasome Subunit Beta Type 5; Recombinant Human Proteasome Subunit Beta Type 5; PSMB5 Human; Proteasome Subunit Beta Type 5 Human Recombinant; Proteasome subunit beta type-5; Macropain epsilon chain; Multicatalytic endopeptidase complex epsilon chain; Proteasome chain 6; Proteasome epsilon chain; Proteasome subunit MB1; Proteasome subunit X; PSMB5; LMPX; MB1; X; MGC104214; PSMB5 recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 90.0% as determined by SDS-PAGE.
• Form/Format: The PSMB5 solution (0.25 mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 20% glycerol, 5mM DTT and 0.2M NaCl.; Sterile Filtered colorless solution.
• Sequence: MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSMTTT LAFKFRHGVI VAADSRATAG AYIASQTVKK VIEINPYLLG TMAGGAADCS FWERLLARQC RIYELRNKER ISVAAASKLL ANMVYQYKGM GLSMGTMICG WDKRGPGLYY VDSEGNRISG ATFSVGSGSV YAYGVMDRGY SYDLEVEQAY DLARRAIYQA TYRDAYSGGA VNLYHVREDG WIRVSSDNVA DLHEKYSGST P
• Sequence Length: 160

• Preparation and Storage: Store at 4 degree C if entire vial will be used within 2-4 weeks. Store, frozen at -20 degree C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles.

Related Product Information for PSMB5 recombinant protein

Description: PSMB5 Human Recombinant fused with a 36 amino acid His tag at N-terminus produced in E Coli is a single, non-glycosylated, polypeptide chain containing 241 amino acids (60-263 a.a.) and having a molecular mass of 26.7kDa. The PSMB5 is purified by proprietary chromatographic techniques.

Introduction: PSMB5 belongs to the proteasome B-type family that has a 20S core beta subunit in the proteasome. This catalytic subunit is not present in the immunoproteasome and is substituted by catalytic subunit 3i (proteasome beta 8 subunit). A fundamental function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. PSMB5 exhibits an ATP dependent proteolytic activity and is involved in an ATP/ubiquitin dependent non lysosomal proteolytic pathway.

Product Categories/Family for PSMB5 recombinant protein

Enzymes; Proteasome

NCBI and Uniprot Product Information

• NCBI GI #: 195539356
• NCBI GeneID: 5693
• NCBI Accession #: NP_001124197.1
• NCBI GenBank Nucleotide #: NM_001130725.1
• UniProt Accession #: P28074; Q16242; Q6PEW2; Q7Z3B5; Q86T01; Q9TNN9; B2R4N9; B4DUM9; D3DS43; E9PAV2
• Molecular Weight: 17,782 Da
• NCBI Official Full Name: proteasome subunit beta type-5 isoform 2
• NCBI Official Synonym Full Names: proteasome (prosome, macropain) subunit, beta type, 5
• NCBI Official Symbol: PSMB5
• NCBI Official Synonym Symbols: X; MB1; LMPX
• NCBI Protein Information: proteasome subunit beta type-5; PSX large multifunctional protease X; macropain epsilon chain; multicatalytic endopeptidase complex epsilon chain; proteasome beta 5 subunit; proteasome catalytic subunit 3; proteasome chain 6; proteasome epsilon chain; proteasome subunit MB1; proteasome subunit X; proteasome subunit, beta type, 5
• UniProt Protein Name: Proteasome subunit beta type-5
• Protein Family: Proteasome
• UniProt Gene Name: PSMB5
• UniProt Synonym Gene Names: LMPX; MB1; X
• UniProt Entry Name: PSB5_HUMAN

NCBI Description

The proteasome is a multicatalytic proteinase complex with a highly ordered ring-shaped 20S core structure. The core structure is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a member of the proteasome B-type family, also known as the T1B family, that is a 20S core beta subunit in the proteasome. This catalytic subunit is not present in the immunoproteasome and is replaced by catalytic subunit 3i (proteasome beta 8 subunit). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2009]

Uniprot Description

PSMB5: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. This unit is responsible of the chymotrypsin-like activity of the proteasome and is one of the principal target of the proteasome inhibitor bortezomib. May catalyze basal processing of intracellular antigens. Plays a role in the protection against oxidative damage through the Nrf2-ARE pathway. Belongs to the peptidase T1B family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; Proteasome complex; EC 3.4.25.1

Chromosomal Location of Human Ortholog: 14q11.2

Cellular Component: proteasome complex; nucleoplasm; proteasome core complex; centrosome; nucleus; cytosol

Molecular Function: threonine endopeptidase activity; protein binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; response to oxidative stress; mitotic cell cycle; regulation of amino acid metabolic process; negative regulation of apoptosis; G1/S transition of mitotic cell cycle