Proteasome Subunit Alpha Type 7 Recombinant Protein | PSMA7 recombinant protein

Recombinant Human Proteasome Subunit Alpha Type 7

Cat. Num. AAA143607

• Gene Names: PSMA7; C6; HSPC; RC6-1; XAPC7
• Purity: Greater than 90.0% as determined SDS-PAGE.
• Synonyms: Proteasome Subunit Alpha Type 7; Recombinant Human Proteasome Subunit Alpha Type 7; PSMA7 Human; Proteasome Subunit Alpha Type 7 Human Recombinant; HSPC; RC6-1; XAPC7; MGC3755; PSMA-7; Proteasome subunit alpha type-7; Proteasome subunit RC6-1; Proteasome subunit XAPC7; PSMA7; C6; PSMA7 recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 90.0% as determined SDS-PAGE.
• Form/Format: The PSMA7 solution contains 20mM Tris-HCl pH-8, 0.1M NaCl, and 20% glycerol.; Sterile Filtered colorless solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MSYDRAITVF SPDGHLFQVE YAQEAVKKGS TAVGVRGRDI VVLGVEKKSV AKLQDERTVR KICALDDNVC MAFAGLTADA RIVINRARVE CQSHRLTVED PVTVEYITRY IASLKQRYTQ SNGRRPFGIS ALIVGFDFDG TPRLYQTDPS GTYHAWKANA IGRGAKSVRE FLEKNYTDEA IETDDLTIKL VIKALLEVVQ SGGKNIELAV MRRDQSLKIL NPEEIEKYVA EIEKEKEENE KKKQKKAS
• Sequence Length: 248

• Preparation and Storage: PSMA7 although stable 4 degree C for 4 weeks, should be stored desiccated below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles.

Related Product Information for PSMA7 recombinant protein

Description: PSMA7 Human Recombinant fused to N-terminal His-Tag produced in E Coli is a single, non-glycosylated polypeptide chain containing 268 amino acids (1-248) and having a molecular mass of 30kDa. PSMA7 is fused to a 20 amino acid His Tag at N-Terminus and purified by standard chromatography techniques.

Introduction: The proteasome is a multicatalytic proteinase complex with a highly assembled ring-shaped 20S core structure which is composed of 4 rings of 28 non-identical subunits, 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are found throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. PSMA7 is part of the peptidase T1A family, that is a 20S core alpha subunit. PSMA7 interacts particularly with the hepatitis B virus X protein, a protein critical to viral replication. PSMA7 is involved in regulating hepatitis virus C internal ribosome entry site activity that is crucial for viral replication. PSMA7 is in charge for regulating the hypoxia-inducible factor-1alpha, a transcription factor important for cellular responses to oxygen tension. PSMA7 is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. PSMA7 interacts specifically with two subdomains of HIF-1alpha and inhibited the transactivation function of HIF-1alpha under both normoxic and hypoxia-mimicking conditions.

Product Categories/Family for PSMA7 recombinant protein

Enzymes; Proteasome

NCBI and Uniprot Product Information

• NCBI GI #: 4506189
• NCBI GeneID: 5688
• NCBI Accession #: NP_002783.1
• NCBI GenBank Nucleotide #: NM_002792.3
• UniProt Accession #: O14818; Q5JXJ2; Q9BR53; Q9H4K5; Q9UEU8; B2R515
• Molecular Weight: 16,135 Da
• NCBI Official Full Name: proteasome subunit alpha type-7
• NCBI Official Synonym Full Names: proteasome (prosome, macropain) subunit, alpha type, 7
• NCBI Official Symbol: PSMA7
• NCBI Official Synonym Symbols: C6; HSPC; RC6-1; XAPC7
• NCBI Protein Information: proteasome subunit alpha type-7; proteasome subunit RC6-1; proteasome subunit XAPC7; proteasome subunit alpha 4
• UniProt Protein Name: Proteasome subunit alpha type-7
• Protein Family: Proteasome
• UniProt Gene Name: PSMA7
• UniProt Synonym Gene Names: HSPC
• UniProt Entry Name: PSA7_HUMAN

NCBI Description

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. This gene encodes a member of the peptidase T1A family that functions as a 20S core alpha subunit. The encoded protein interacts with the hepatitis B virus X protein and plays a role in regulating hepatitis C virus internal ribosome entry site (IRES) activity, an activity essential for viral replication. The encoded protein also plays a role in the cellular stress response by regulating hypoxia-inducible factor-1alpha. A pseudogene of this gene is located on the long arm of chromosome 9. [provided by RefSeq, Jul 2012]

Uniprot Description

PSMA7: The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity. Plays an important role in the regulation of cell proliferation or cell cycle control, transcriptional regulation, immune and stress response, cell differentiation, and apoptosis. Interacts with some important proteins involved in transcription factor regulation, cell cycle transition, viral replication and even tumor initiation and progression. Inhibits the transactivation function of HIF-1A under both normoxic and hypoxia-mimicking conditions. The interaction with EMAP2 increases the proteasome-mediated HIF-1A degradation under the hypoxic conditions. Plays a role in hepatitis C virus internal ribosome entry site-mediated translation. Mediates nuclear translocation of the androgen receptor (AR) and thereby enhances androgen-mediated transactivation. Promotes MAVS degradation and thereby negatively regulates MAVS-mediated innate immune response. The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel. PSMA7 interacts directly with the PSMG1-PSMG2 heterodimer which promotes 20S proteasome assembly. Interacts with HIV-1 TAT protein. Interacts with hepatitis B virus X protein (HBX). Interacts with HIF1A. Interacts with RAB7A. Interacts with PARK2. Interacts with ABL1 and ABL2. Interacts with EMAP2. Interacts with MAVS. Down-regulated by the ribozyme Rz3'X. Up-regulated in colorectal cancer tissues. Belongs to the peptidase T1A family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Protease; Proteasome complex; EC 3.4.25.1

Chromosomal Location of Human Ortholog: 20q13.33

Cellular Component: nucleoplasm; proteasome complex; proteasome core complex; nucleus; cytosol

Molecular Function: identical protein binding; threonine endopeptidase activity; protein binding

Biological Process: positive regulation of ubiquitin-protein ligase activity during mitotic cell cycle; negative regulation of ubiquitin-protein ligase activity during mitotic cell cycle; protein polyubiquitination; viral reproduction; apoptosis; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest; regulation of apoptosis; antigen processing and presentation of peptide antigen via MHC class I; regulation of ubiquitin-protein ligase activity during mitotic cell cycle; anaphase-promoting complex-dependent proteasomal ubiquitin-dependent protein catabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I; gene expression; mitotic cell cycle; regulation of amino acid metabolic process; G1/S transition of mitotic cell cycle; negative regulation of apoptosis