Mouse PC4 and SFRS1-interacting protein ELISA Kit | Psip1 elisa kit

Mouse PC4 and SFRS1-interacting protein ELISA Kit

Cat. Num. AAA288345

• Gene Names: Psip1; Dfs70; Psip2; Ledgfa; Ledgfb; AA408851; AU015605
• Reactivity: Mouse
• Synonyms: PC4 and SFRS1-interacting protein; Mouse PC4 and SFRS1-interacting protein ELISA Kit; Psip1 elisa kit

• Reactivity: Mouse
• Specificity: This assay recognizes recombinant and natural mouse PC4 and SFRS1-interacting protein. No significant cross-reactivity or interference was observed.
• Sequence Length: 530

• Assay Type: Sandwich
• Detection Range: 0.156-10 ng/ml
• Sensitivity: 0.095 ng/mL
• Intra-Assay CV: <=6.4%
• Inter-Assay CV: <=8.9%
• Recovery: 86%
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Psip1 elisa kit

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PC4 and SFRS1-interacting protein. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for PC4 and SFRS1-interacting protein and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain PC4 and SFRS1-interacting protein, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of PC4 and SFRS1-interacting protein in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 594542483
• NCBI GeneID: 101739
• NCBI Accession #: NP_001277456.1
• NCBI GenBank Nucleotide #: NM_001290527.1
• UniProt Accession #: Q99JF8; Q3TEJ7; Q3TTD7; Q3UTA1; Q80WQ7; Q99JF7; Q99LR4; Q9CT03; A2BI10; A2BI11
• Molecular Weight: 37,426 Da
• NCBI Official Full Name: PC4 and SFRS1-interacting protein isoform 2
• NCBI Official Synonym Full Names: PC4 and SFRS1 interacting protein 1
• NCBI Official Symbol: Psip1
• NCBI Official Synonym Symbols: Dfs70; Psip2; Ledgfa; Ledgfb; AA408851; AU015605
• NCBI Protein Information: PC4 and SFRS1-interacting protein
• UniProt Protein Name: PC4 and SFRS1-interacting protein
• Protein Family: PC4 and SFRS1-interacting protein
• UniProt Gene Name: Psip1
• UniProt Synonym Gene Names: Ledgf; mLEDGF
• UniProt Entry Name: PSIP1_MOUSE

Uniprot Description

LEDGF: Transcriptional coactivator involved in neuroepithelial stem cell differentiation and neurogenesis. Involved in particular in lens epithelial cell gene regulation and stress responses. May play an important role in lens epithelial to fiber cell terminal differentiation. May play a protective role during stress-induced apoptosis. Isoform 2 is a more general and stronger transcriptional coactivator. Isoform 2 may also act as an adapter to coordinate pre-mRNA splicing. Cellular cofactor for lentiviral integration. Monomer. Interacts with IFRD1/PC4. Isoform 2 interacts with SFRS1. Isoform 1 interacts POGZ and CDCA7L. Isoform 1 interacts with lentiviral integrase and acts as a chromatin tethering factor to the chromosomal DNA. Isoform 1 interacts in particular with the human HIV-1 integrase protein (HIV-1 IN), determining its nuclear localization, its tight association with chromatin and its protection from the proteasome. Isoform 1 interacts also with HIV-2 IN. Isoform 2 does not interact with HIV-1 IN. Widely expressed. Expressed at high level in the thymus. Expressed in fetal and adult brain. Expressed in neurons, but not astrocytes. Markedly elevated in fetal as compared to adult brain. In the adult brain, expressed in the subventricular zone (SVZ), in hippocampus, and undetectable elsewhere. In the fetal brain, expressed in the germinal neuroepithelium and cortical plate regions. Belongs to the HDGF family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription, coactivator/corepressor; Motility/polarity/chemotaxis

Cellular Component: nuclear heterochromatin; nucleoplasm; nucleus

Molecular Function: chromatin binding; DNA binding; transcription activator binding

Biological Process: nuclear mRNA 5'-splice site recognition; positive regulation of transcription from RNA polymerase II promoter; regulation of transcription, DNA-dependent; response to heat; response to oxidative stress; transcription, DNA-dependent