Human Pre-mRNA-processing-splicing factor 8 ELISA Kit | PRPF8 elisa kit
Human Pre-mRNA-processing-splicing factor 8 (PRPF8) ELISA Kit
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PRPF8 in Human serum, plasma, tissue homogenates. An antibody specific for PRPF8 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PRPF8 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRPF8 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRPF8 bound in the initial step. The color development is stopped and the intensity of the color is measured.
NCBI and Uniprot Product Information
NCBI Description
Pre-mRNA splicing occurs in 2 sequential transesterification steps. The protein encoded by this gene is a component of both U2- and U12-dependent spliceosomes, and found to be essential for the catalytic step II in pre-mRNA splicing process. It contains several WD repeats, which function in protein-protein interactions. This protein has a sequence similarity to yeast Prp8 protein. This gene is a candidate gene for autosomal dominant retinitis pigmentosa. [provided by RefSeq, Jul 2008]
Uniprot Description
PRPF8: Central component of the spliceosome, which may play a role in aligning the pre-mRNA 5'- and 3'-exons for ligation. Interacts with U5 snRNA, and with pre-mRNA 5'-splice sites in B spliceosomes and 3'-splice sites in C spliceosomes. Defects in PRPF8 are the cause of retinitis pigmentosa type 13 (RP13). RP leads to degeneration of retinal photoreceptor cells. Patients typically have night vision blindness and loss of midperipheral visual field. As their condition progresses, they lose their far peripheral visual field and eventually central vision as well. RP13 inheritance is autosomal dominant.
Protein type: RNA splicing; RNA-binding; Spliceosome
Chromosomal Location of Human Ortholog: 17p13.3
Cellular Component: catalytic step 2 spliceosome; membrane; nuclear speck; nucleoplasm; nucleus; snRNP U5; U4/U6 x U5 tri-snRNP complex
Molecular Function: protein binding; RNA binding; second spliceosomal transesterification activity; U1 snRNA binding; U2 snRNA binding; U5 snRNA binding; U6 snRNA binding
Biological Process: cellular response to tumor necrosis factor; mRNA processing; mRNA splicing, via spliceosome; RNA splicing; RNA splicing, via transesterification reactions; spliceosomal tri-snRNP complex assembly
Disease: Retinitis Pigmentosa 13
Research Articles on PRPF8
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Product Notes
The Human PRPF8 prpf8 (Catalog #AAA283058) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA283058 ELISA Kit recognizes Human PRPF8. It is sometimes possible for the material contained within the vial of "Pre-mRNA-processing-splicing factor 8, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
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