Pre-mRNA-processing factor 19 (Prpf19) Recombinant Protein | Prpf19 recombinant protein

Recombinant Rat Pre-mRNA-processing factor 19 (Prpf19)

Cat. Num. AAA1360680

• Gene Names: Prpf19; Prp19
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Pre-mRNA-processing factor 19 (Prpf19); Recombinant Rat Pre-mRNA-processing factor 19 (Prpf19); Prpf19 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Positions: 2-504, full length protein
• Sequence: SLICSISNEVPEHPCVSPVSNHVYERRLIEKYIAENGTDPINNQPLSEEQLIDIKVAHPIRPKPPSATSIPAILKALQDEWDAVMLHSFTLRQQLQTTRQELSHALYQHDAACRVIARLTKEVTAAREALATLKPQAGLIVPQAVPSSQPSVVGAGEPMDLGELVGMTPEIIQKLQDKATVLTTERKKRGKTVPEELVKPEELSKYRQVASHVGLHSASIPGILALDLCPSDTNKILTGGADKNVVVFDKSTEQILATLKGHTKKVTSVVFHPSQELVFSASPDATIRIWSVPNTSCVQVVRAHESAVTGLSLHATGDYLLSSSDDQYWAFSDIQTGRVLTKVTDETSGCSLTCAQFHPDGLIFGTGTMDSQIKIWDLKERTNVANFPGHSGPITSIAFSENGYYLATAADDSSVKLWDLRKLKNFKTLQLDNNFEVKSLIFDQSGTYLALGGTDVQIYICKQWTEILHFTEHSGLTTGVAFGHHAKFIASTGMDRSLKFYSL
• Sequence Length: 503

• Species: Rat
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 74095899
• NCBI GeneID: 246216
• NCBI Accession #: NP_647549.2
• NCBI GenBank Nucleotide #: NM_139333.3
• UniProt Accession #: Q9JMJ4; Q3B7C6
• Molecular Weight: 55,239 Da
• NCBI Official Full Name: pre-mRNA-processing factor 19
• NCBI Official Synonym Full Names: pre-mRNA processing factor 19
• NCBI Official Symbol: Prpf19
• NCBI Official Synonym Symbols: Prp19
• NCBI Protein Information: pre-mRNA-processing factor 19
• UniProt Protein Name: Pre-mRNA-processing factor 19
• UniProt Gene Name: Prpf19

NCBI Description

human homolog is involved in DNA double strand break repair [RGD, Feb 2006]

Uniprot Description

Ubiquitin-protein ligase which is a core component of several complexes mainly involved pre-mRNA splicing and DNA repair. Core component of the PRP19C/Prp19 complex/NTC/Nineteen complex which is part of the spliceosome and participates in its assembly, its remodeling and is required for its activity. During assembly of the spliceosome, mediates 'Lys-63'-linked polyubiquitination of the U4 spliceosomal protein PRPF3. Ubiquitination of PRPF3 allows its recognition by the U5 component PRPF8 and stabilizes the U4/U5/U6 tri-snRNP spliceosomal complex. Recruited to RNA polymerase II C-terminal domain (CTD) and the pre-mRNA, it may also couple the transcriptional and spliceosomal machineries. The XAB2 complex, which contains PRPF19, is also involved in pre-mRNA splicing, transcription and transcription-coupled repair. Beside its role in pre-mRNA splicing PRPF19, as part of the PRP19-CDC5L complex, plays a role in the DNA damage response/DDR. It is recruited to the sites of DNA damage by the RPA complex where PRPF19 directly ubiquitinates RPA1 and RPA2. 'Lys-63'-linked polyubiquitination of the RPA complex allows the recruitment of the ATR-ATRIP complex and the activation of ATR, a master regulator of the DNA damage response. May also play a role in DNA double-strand break (DSB) repair by recruiting the repair factor SETMAR to altered DNA. As part of the PSO4 complex may also be involved in the DNA interstrand cross-links/ICLs repair process. In addition, may also mediate 'Lys-48'-linked polyubiquitination of substrates and play a role in proteasomal degradation (). May play a role in the biogenesis of lipid droplets (). May play a role in neural differentiation possibly through its function as part of the spliceosome (PubMed:16352598).