Protein Kinase C, alpha Protein | PRKCA protein

Protein Kinase C, alpha, phosphorylated (Ser-657/Tyr-658), Control Peptide (AAG6, MGC129900, MGC129901, PKCA, PKC-A, PKC-alpha, PRKACA, Protein kinase C alpha type)

Cat. Num. AAA634495

• Gene Names: PRKCA; AAG6; PKCA; PRKACA; PKC-alpha
• Applications: ELISA
• Purity: Purified; Purified
• Synonyms: Protein Kinase C; alpha; phosphorylated (Ser-657/Tyr-658); Control Peptide (AAG6; MGC129900; MGC129901; PKCA; PKC-A; PKC-alpha; PRKACA; Protein kinase C alpha type); PRKCA protein

• Purity/Purification: Purified; Purified
• Form/Format: Supplied as a liquid in PBS with 0.05% sodium azide.

• Applicable Applications for PRKCA protein: ELISA (EL/EIA)
• Application Notes: Suitable for use in ELISA or for antigen applications in immunological protocols.; Dilution: End user should determine optimal dilution for their particular applications and experiments. These peptides quantities were established in 96-well plates where the peptide was bound to plates for 2 hrs in 0.1M sodium carbonate buffer, pH 8.5.
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Product Categories/Family for PRKCA protein

Molecular Biology; MB-Protein Kinases

NCBI and Uniprot Product Information

• NCBI GI #: 119609420
• NCBI GeneID: 5578
• UniProt Accession #: P17252; Q15137; Q32M72; Q96RE4; B5BU22
• Molecular Weight: 76,750 Da
• NCBI Official Full Name: protein kinase C, alpha
• NCBI Official Synonym Full Names: protein kinase C, alpha
• NCBI Official Symbol: PRKCA
• NCBI Official Synonym Symbols: AAG6; PKCA; PRKACA; PKC-alpha
• NCBI Protein Information: protein kinase C alpha type; PKC-A; aging-associated gene 6
• UniProt Protein Name: Protein kinase C alpha type
• Protein Family: Protein kinase
• UniProt Gene Name: PRKCA
• UniProt Synonym Gene Names: PKCA; PRKACA; PKC-A; PKC-alpha
• UniProt Entry Name: KPCA_HUMAN

NCBI Description

Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. PKC family members also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role in cells. The protein encoded by this gene is one of the PKC family members. This kinase has been reported to play roles in many different cellular processes, such as cell adhesion, cell transformation, cell cycle checkpoint, and cell volume control. Knockout studies in mice suggest that this kinase may be a fundamental regulator of cardiac contractility and Ca(2+) handling in myocytes. [provided by RefSeq, Jul 2008]

Uniprot Description

PKCA: an AGC kinase of the PKC family. A classical PKC downstream of many mitogenic and receptors. Classical PKCs are calcium-dependent enzymes that are activated by phosphatidylserine, diacylglycerol and phorbol esters. Contains a pseudo-substrate autoinhibitory domain that binds to the catalytic domain preventing its activation in the absence of cofactors or activators.

Protein type: Protein kinase, AGC; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.13; Oncoprotein; AGC group; PKC family; Alpha subfamily

Chromosomal Location of Human Ortholog: 17q22-q23.2

Cellular Component: nucleoplasm; photoreceptor outer segment; cell soma; mitochondrion; perinuclear region of cytoplasm; apical part of cell; endoplasmic reticulum; mitochondrial membrane; cytoplasm; dendrite; plasma membrane; cytosol

Molecular Function: protein serine/threonine kinase activity; protein binding; enzyme binding; protein kinase C activity; zinc ion binding; calcium-dependent protein kinase C activity; ATP binding; protein kinase activity

Biological Process: phototransduction, visible light; extracellular matrix organization and biogenesis; positive regulation of cell adhesion; nerve growth factor receptor signaling pathway; regulation of muscle contraction; mitotic nuclear envelope disassembly; positive regulation of lipopolysaccharide-mediated signaling pathway; negative regulation of insulin receptor signaling pathway; positive regulation of mitotic cell cycle; signal transduction; protein amino acid phosphorylation; induction of positive chemotaxis; synaptic transmission; negative regulation of cell proliferation; chondrocyte differentiation; angiogenesis; cell adhesion; positive regulation of macrophage differentiation; regulation of peptidyl-tyrosine phosphorylation; regulation of the force of heart contraction; inactivation of MAPK activity; epidermal growth factor receptor signaling pathway; platelet activation; neutrophil chemotaxis; fibroblast growth factor receptor signaling pathway; negative regulation of adenylate cyclase activity; adenylate cyclase activation; positive regulation of blood vessel endothelial cell migration; cellular calcium ion homeostasis; rhodopsin mediated signaling; negative regulation of glucose import; positive regulation of angiogenesis; regulation of rhodopsin mediated signaling; phospholipase C activation; energy reserve metabolic process; innate immune response; positive regulation of endothelial cell proliferation; gene expression; positive regulation of protein amino acid phosphorylation; mitotic cell cycle; vascular endothelial growth factor receptor signaling pathway; blood coagulation; regulation of insulin secretion; positive regulation of inflammatory response; positive regulation of cell migration