Limulus Anti-LPS Factor Peptide Protein
Limulus Anti-LPS Factor Peptide (LALF)
Applications
ELISA
Purity
Purified
Synonyms
Limulus Anti-LPS Factor Peptide; Limulus Anti-LPS Factor Peptide (LALF); Limulus Anti-LPS Factor Peptide protein
Purity/Purification
Purified
Form/Format
Supplied as a lyophilized powder in PBS. Reconstitute with sterile, endotoxin free PBS
Applicable Applications for Limulus Anti-LPS Factor Peptide protein
ELISA (EL/EIA)
Application Notes
Suitable for use in biological assays in vivo and in vitro. Dilutions have to be made according to the amount of protein to be inactivated. Further dilutions should be made by using protein stabilized PBS, pH 7 (never use fresh serum/plasma). Be aware: Endotoxin will bind LALF-peptide.
Optimal dilutions to be determined by the researcher.
Optimal dilutions to be determined by the researcher.
Inactivation
For neutralization of biological activity dilutions have to be made according to the amounts protein to be inactivated. Further dilutions should be made by using protein stabilized phosphate buffered saline, pH7. (Never use fresh serum/plasma). Be aware: endotoxin will bind LALF-peptide.
Preparation and Storage
Lyophilized powder may be stored at 4°C and is stable for 12 months at 4°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Stock solution should be stored in aliquots at -70°C in polypropylene ntubes. LALF-peptide stock solution should never be thawed more than oce. Repeated freeze and thaw cycles will cause loss of LALF-peptide activity.
Related Product Information for Limulus Anti-LPS Factor Peptide protein
Description: LALF-peptide (amino acids 31-52) from horseshoe crab Limulus anti-LPS factor protein
The name of "Limulus" is derived from a scientific name of the horseshoe crab; Limulus polyphemus. Four species of horseshoe crabs exist in limited regions of the world. The hemolymph amebocyte is the circulating blood cell of the horseshoe crab. Levin and Bang found out that endotoxin (LPS) induces coagulation of the lysate of hemolymph caused by an anti-LPS factor. Limulus anti-LPS factor (LALF) is a naturally occurring horseshoe crab derived protein that is both bactericidal for gram-negative bacteria and capable of neutralizing LPS. The cyclic LALF-peptide, amino acids 31-52, binds lipid A and neutralizes LPS in vivo and in vitro.
The name of "Limulus" is derived from a scientific name of the horseshoe crab; Limulus polyphemus. Four species of horseshoe crabs exist in limited regions of the world. The hemolymph amebocyte is the circulating blood cell of the horseshoe crab. Levin and Bang found out that endotoxin (LPS) induces coagulation of the lysate of hemolymph caused by an anti-LPS factor. Limulus anti-LPS factor (LALF) is a naturally occurring horseshoe crab derived protein that is both bactericidal for gram-negative bacteria and capable of neutralizing LPS. The cyclic LALF-peptide, amino acids 31-52, binds lipid A and neutralizes LPS in vivo and in vitro.
Product Categories/Family for Limulus Anti-LPS Factor Peptide protein
NCBI and Uniprot Product Information
UniProt Accession #
Similar Products
Product Notes
The Limulus Anti-LPS Factor Peptide (Catalog #AAA634822) is a Protein and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Limulus Anti-LPS Factor Peptide can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA). Suitable for use in biological assays in vivo and in vitro. Dilutions have to be made according to the amount of protein to be inactivated. Further dilutions should be made by using protein stabilized PBS, pH 7 (never use fresh serum/plasma). Be aware: Endotoxin will bind LALF-peptide. Optimal dilutions to be determined by the researcher. Researchers should empirically determine the suitability of the Limulus Anti-LPS Factor Peptide for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Limulus Anti-LPS Factor Peptide, Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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