Protein A/G Recombinant Protein
Protein A/G, Recombinant (Biotin)
Purity
Highly Purified98% (SDS-PAGE and HPLC)
Synonyms
Protein A/G; Recombinant (Biotin); Protein A/G recombinant protein
Host
E Coli
Specificity
Under optimal conditions, 1mg protein A/G will bind approximately 5mg human IgG. Optimal binding of Protein A/G to antibodies occurs at pH 5.0 to 8.0 and can be eluted over a pH range of 2.5 to 3.0
Purity/Purification
Highly Purified
98% (SDS-PAGE and HPLC)
98% (SDS-PAGE and HPLC)
Form/Format
Supplied as a lyophilized powder. Labeled with Biotin. Reconstitute with 20ul sterile ddH2O.
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for Protein A/G recombinant protein
Protein A/G is a genetically engineered protein that combines the IgG binding profiles of both Protein A and Protein G. It is a gene fusion product. Recombinant fusion protein A/G contains 6x His-tag on the N-terminus, five Ig-binding regions of protein A fusion with three Ig-binding region of protein G. Cell wall binding region, albumin binding region and other non-specific binding regions have been eliminated from the fusion protein A/G to ensure the maximum specific IgG binding. 6x His-tag on N-terminus can be used for affinity purification or for protein A/G detection using anti-His-tag antibody.
Protein A/G binds to all IgG subclasses from various mammalian species, including all IgGs that bind to both Protein A and Protein G.
Protein A/G-Biotin is prepared by conjugating recombinant Protein A/G to Biotin. The conjugation was optimized to provide the maximal binding capacity for IgG.
Protein A/G binds to all IgG subclasses from various mammalian species, including all IgGs that bind to both Protein A and Protein G.
Protein A/G-Biotin is prepared by conjugating recombinant Protein A/G to Biotin. The conjugation was optimized to provide the maximal binding capacity for IgG.
Product Categories/Family for Protein A/G recombinant protein
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
59.7kD
NCBI Official Full Name
putative outer membrane protein A (3a;II*;G;d)
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Product Notes
The Protein A/G (Catalog #AAA637308) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Protein A/G, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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