Cat Major prion protein ELISA Kit | PRNP elisa kit

Cat Major prion protein (PRNP) ELISA Kit

Cat. Num. AAA285466

• Gene Names: PRNP; PRP
• Reactivity: Cat
• Synonyms: Major prion protein; Cat Major prion protein (PRNP) ELISA Kit; ASCR; CD230; CJD; GSS; MGC26679; PRIP; PrP; PrP27-30; PrP33-35C; PrPc; prion; CD230 antigen|major prion protein|p27-30|prion protein PrP|prion-related protein; PRNP elisa kit

• Reactivity: Cat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Cat PRNP. No significant cross-reactivity or interference between Cat PRNP and analogues was observed.
• Sequence Length: 253

• Samples: Serum, Plasma, Other biological fluids
• Assay Type: Sandwich
• Sample Volume: 1-200 uL
• Precision: Intra-assay Precision (Precision within an assay); Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays); Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for PRNP elisa kit

Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitate PRNP in samples. An antibody specific for PRNP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and anyPRNP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PRNP is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PRNP bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 83305348
• NCBI GeneID: 101087310
• UniProt Accession #: O18754; O19016; Q5YCW7
• Molecular Weight: 27,968 Da
• NCBI Official Full Name: Major prion protein
• NCBI Official Symbol: PRNP
• NCBI Official Synonym Symbols: PRP
• NCBI Protein Information: major prion protein
• UniProt Protein Name: Major prion protein
• Protein Family: Major prion protein
• UniProt Gene Name: PRNP
• UniProt Synonym Gene Names: PRP; PrP

Uniprot Description

Its primary physiological function is unclear. May play a role in neuronal development and synaptic plasticity. May be required for neuronal myelin sheath maintenance. May promote myelin homeostasis through acting as a agonist for ADGRG6 receptor. May play a role in iron uptake and iron homeostasis. Soluble oligomers are toxic to cultured neuroblastoma cells and induce apoptosis (in vitro) (). Association with GPC1 (via its heparan sulfate chains) targets PRNP to lipid rafts. Also provides Cu2+ or ZN2+ for the ascorbate-mediated GPC1 deaminase degradation of its heparan sulfate side chains ().