Mouse Prion-like protein doppel ELISA Kit | Prnd elisa kit

Mouse Prion-like protein doppel ELISA Kit

Cat. Num. AAA761468

• Gene Names: PRND; DPL; PrPLP; DOPPEL; dJ1068H6.4
• Reactivity: Mouse
• Synonyms: Prion-like protein doppel; Mouse Prion-like protein doppel ELISA Kit; Prnd/PRND (Prion-like protein doppel)/DPL/Dublet/PrPLP/Prion protein 2; Prnd elisa kit

• Reactivity: Mouse
• Sequence Length: 176

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 78-5000pg/ml
• Sensitivity: 46.9pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Prnd elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-Mouse Prnd antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Prnd antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Prnd amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Prnd can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 34335268
• NCBI GeneID: 23627
• NCBI Accession #: NP_036541.2
• NCBI GenBank Nucleotide #: NM_012409.3
• Molecular Weight: 20,293 Da
• NCBI Official Full Name: prion-like protein doppel preproprotein
• NCBI Official Synonym Full Names: prion protein 2 (dublet)
• NCBI Official Symbol: PRND
• NCBI Official Synonym Symbols: DPL; PrPLP; DOPPEL; dJ1068H6.4
• NCBI Protein Information: prion-like protein doppel
• UniProt Protein Name: Prion-like protein doppel
• Protein Family: Prion-like protein doppel
• UniProt Gene Name: PRND
• UniProt Synonym Gene Names: DPL
• UniProt Entry Name: PRND_HUMAN

NCBI Description

This gene is found on chromosome 20, approximately 20 kbp downstream of the gene encoding cellular prion protein, to which it is biochemically and structurally similar. The protein encoded by this gene is a membrane glycosylphosphatidylinositol-anchored glycoprotein that is found predominantly in testis. Mutations in this gene may lead to neurological disorders. [provided by RefSeq, Jul 2008]

Uniprot Description

PRND: This gene is found on chromosome 20, approximately 20 kbp downstream of the gene encoding cellular prion protein, to which it is biochemically and structurally similar. The protein encoded by this gene is a membrane glycosylphosphatidylinositol-anchored glycoprotein that is found predominantly in testis. Mutations in this gene may lead to neurological disorders. [provided by RefSeq, Jul 2008]

Protein type: Membrane protein, GPI anchor

Chromosomal Location of Human Ortholog: 20p13

Cellular Component: plasma membrane

Molecular Function: copper ion binding

Biological Process: cellular copper ion homeostasis; protein homooligomerization