Protein arginine N-methyltransferase 5 (PRMT5) Recombinant Protein | PRMT5 recombinant protein

Recombinant Macaca fascicularis Protein arginine N-methyltransferase 5 (PRMT5), partial

• Gene Names: PRMT5; SKB1
• Purity: Greater or equal to 85% purity as determined by SDS-PAGE.
• Synonyms: Protein arginine N-methyltransferase 5 (PRMT5); Recombinant Macaca fascicularis Protein arginine N-methyltransferase 5 (PRMT5); partial; PRMT5 recombinant protein

• Host: E Coli or Yeast or Baculovirus or Mammalian Cell
• Purity/Purification: Greater or equal to 85% purity as determined by SDS-PAGE.
• Form/Format: Lyophilized or liquid (Format to be determined during the manufacturing process)
• Sequence Length: 637

• Species: Macaca fascicularis (Crab-eating macaque) (Cynomolgus monkey)
• Preparation and Storage: Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

• NCBI GI #: 82581643
• NCBI GeneID: 101866818
• UniProt Accession #: Q4R5M3
• Molecular Weight: 72,566 Da
• NCBI Official Full Name: Protein arginine N-methyltransferase 5
• NCBI Official Symbol: PRMT5
• NCBI Official Synonym Symbols: SKB1
• NCBI Protein Information: protein arginine N-methyltransferase 5
• UniProt Protein Name: Protein arginine N-methyltransferase 5
• Protein Family: Protein arginine N-methyltransferase
• UniProt Gene Name: PRMT5
• UniProt Synonym Gene Names: SKB1; SKB1 homolog

Uniprot Description

Arginine methyltransferase that can both catalyze the formation of omega-N monomethylarginine (MMA) and symmetrical dimethylarginine (sDMA), with a preference for the formation of MMA. Specifically mediates the symmetrical dimethylation of arginine residues in the small nuclear ribonucleoproteins Sm D1 (SNRPD1) and Sm D3 (SNRPD3); such methylation being required for the assembly and biogenesis of snRNP core particles. Methylates SUPT5H and may regulate its transcriptional elongation properties. Mono- and dimethylates arginine residues of myelin basic protein (MBP) in vitro. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. Methylates histone H2A and H4 'Arg-3' during germ cell development. Methylates histone H3 'Arg-8', which may repress transcription. Methylates the Piwi proteins (PIWIL1, PIWIL2 and PIWIL4), methylation of Piwi proteins being required for the interaction with Tudor domain-containing proteins and subsequent localization to the meiotic nuage. Methylates RPS10. Attenuates EGF signaling through the MAPK1/MAPK3 pathway acting at 2 levels. First, monomethylates EGFR; this enhances EGFR 'Tyr-1197' phosphorylation and PTPN6 recruitment, eventually leading to reduced SOS1 phosphorylation. Second, methylates RAF1 and probably BRAF, hence destabilizing these 2 signaling proteins and reducing their catalytic activity. Required for induction of E-selectin and VCAM-1, on the endothelial cells surface at sites of inflammation. Methylates HOXA9. Methylates and regulates SRGAP2 which is involved in cell migration and differentiation. Acts as a transcriptional corepressor in CRY1-mediated repression of the core circadian component PER1 by regulating the H4R3 dimethylation at the PER1 promoter. Methylates GM130/GOLGA2, regulating Golgi ribbon formation. Methylates H4R3 in genes involved in glioblastomagenesis in a CHTOP- and/or TET1-dependent manner. Symmetrically methylates POLR2A, a modification that allows the recruitment to POLR2A of proteins including SMN1/SMN2 and SETX. This is required for resolving RNA-DNA hybrids created by RNA polymerase II, that form R-loop in transcription terminal regions, an important step in proper transcription termination (). Along with LYAR, binds the promoter of gamma-globin HBG1/HBG2 and represses its expression ().