Mouse protein arginine methyltransferase 5 ELISA Kit | PRMT5 elisa kit

Mouse Protein arginine N-methyltransferase 5, PRMT5 ELISA Kit

Cat. Num. AAA9323188

• Gene Names: Prmt5; Jbp1; Skb1
• Reactivity: Mouse
• Synonyms: protein arginine methyltransferase 5; Mouse Protein arginine N-methyltransferase 5; PRMT5 ELISA Kit; Mouse Protein arginine N-methyltransferase 5 (PRMT5) ELISA kit; HRMT1L5; IBP72; JBP1; SKB1; SKB1Hs; HMT1 hnRNP methyltransferase-like 5; SKB1 homolog; PRMT5 elisa kit

• Reactivity: Mouse

• Assay Type: Quantitative Sandwich
• Detection Range: 0.625ng/ml-20ng/ml
• Sensitivity: 0.1ng/ml
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]. All CV% should be compared by concentration, not compared by OD values.
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for PRMT5 elisa kit

Background/Introduction: This Quantitative Sandwich ELISA kit is for lab reagent/research use only, not for drug, household, therapeutic or test applications! This kit is intended to be used for determine the level of PRMT5 (hereafter termed "analyte") in undiluted original Mouse serum, plasma or tissue homogenates samples. For other sample types please contact tech support to determine compatibility with this assay. This kit is not suitable for assaying non-biological sources of substances.

NCBI and Uniprot Product Information

• NCBI GI #: 188528624
• NCBI GeneID: 27374
• NCBI Accession #: NP_038796.2
• NCBI GenBank Nucleotide #: NM_013768.3
• UniProt Accession #: Q8CIG8; Q3TNN1; Q9QZS9
• Molecular Weight: 72,680 Da
• NCBI Official Full Name: protein arginine N-methyltransferase 5
• NCBI Official Synonym Full Names: protein arginine N-methyltransferase 5
• NCBI Official Symbol: Prmt5
• NCBI Official Synonym Symbols: Jbp1; Skb1
• NCBI Protein Information: protein arginine N-methyltransferase 5; SKB1 homolog; Jak-binding protein 1; shk1 kinase-binding protein 1 homolog; histone-arginine N-methyltransferase PRMT5
• UniProt Protein Name: Protein arginine N-methyltransferase 5
• Protein Family: Protein arginine N-methyltransferase
• UniProt Gene Name: Prmt5
• UniProt Synonym Gene Names: Jbp1; Skb1; SKB1 homolog
• UniProt Entry Name: ANM5_MOUSE

NCBI Description

This gene encodes an enzyme that belongs to the methyltransferase family. The encoded protein catalyzes the transfer of methyl groups to the amino acid arginine, in target proteins that include histones, transcriptional elongation factors and the tumor suppressor p53. This gene plays a role in several cellular processes, including transcriptional regulation and the assembly of small nuclear ribonucleoproteins. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2015]

Uniprot Description

PRMT5: an ubiquitous enzyme with protein methyltransferase activity. Methylates specific arginine residues in the transcription elongation factor SPT5, and the small nuclear ribonucleoproteins SNRPD1 and SNRPD3. Plays a role in the assembly of snRNP core particles. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. May regulate the SPT5 transcriptional elongation properties. May be part of a pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement. Component of the methylosome, a 20S complex that includes ICLN, SKB1 and WDR77. Component of a high molecular weight E2F-pocket protein complex, CERC (cyclin E1 repressor complex). Also interacts with Sm proteins, JAK2, SSTR1 and SPT5. Associates with SWI/SNF remodeling complexes containing SMARCA2 and SMARCA4.

Protein type: RNA processing; EC 2.1.1.125; Methyltransferase; Methyltransferase, protein arginine; Cell cycle regulation

Cellular Component: protein complex; cytoplasm; nucleus; cytosol

Molecular Function: methyltransferase activity; trimethylamine methyltransferase activity; monomethylamine methyltransferase activity; RNA methyltransferase activity; tRNA (cytosine)-methyltransferase activity; rRNA (cytosine-C5-967)-methyltransferase activity; rRNA (uridine) methyltransferase activity; N-methyltransferase activity; protein complex binding; tRNA (guanine) methyltransferase activity; rRNA (adenine-N6,N6-)-dimethyltransferase activity; arginine N-methyltransferase activity; ribonucleoprotein binding; selenocysteine methyltransferase activity; protein-arginine N-methyltransferase activity; methanol-specific methylcobalamin:coenzyme M methyltransferase activity; tRNA (guanine-N2-)-methyltransferase activity; dimethylamine methyltransferase activity; protein methyltransferase activity; 2-polyprenyl-6-methoxy-1,4-benzoquinone methyltransferase activity; transferase activity; hydroxyneurosporene-O-methyltransferase activity; rRNA (uridine-2'-O-)-methyltransferase activity; protein heterodimerization activity; tRNA (uracil) methyltransferase activity; rRNA (adenine) methyltransferase activity; C-methyltransferase activity; chromatin binding; transcription corepressor activity; cobalt-precorrin-6B C5-methyltransferase activity; cobalt-precorrin-3 C17-methyltransferase activity; 1-phenanthrol methyltransferase activity; S-methyltransferase activity; lysine N-methyltransferase activity; DNA-methyltransferase activity; tRNA (cytosine-5-)-methyltransferase activity; tRNA (adenine)-methyltransferase activity; C-terminal protein carboxyl methyltransferase activity; rRNA (cytosine) methyltransferase activity; cobalt-precorrin-7 C15-methyltransferase activity; protein-arginine N5-methyltransferase activity; rRNA methyltransferase activity; protein-leucine O-methyltransferase activity; histone-arginine N-methyltransferase activity; demethylmenaquinone methyltransferase activity; rRNA (adenine-N6-)-methyltransferase activity; tRNA methyltransferase activity; rRNA (guanine) methyltransferase activity; methylarsonite methyltransferase activity; mRNA methyltransferase activity; protein binding; methylamine-specific methylcobalamin:coenzyme M methyltransferase activity; tRNA (guanine-N1-)-methyltransferase activity; cobalt-precorrin-5B C1-methyltransferase activity; protein-arginine omega-N symmetric methyltransferase activity; protein-lysine N-methyltransferase activity; O-methyltransferase activity; tRNA (adenine-N1-)-methyltransferase activity; tRNA (adenine-57, 58-N(1)-) methyltransferase activity

Biological Process: methylation; peptidyl-arginine methylation, to symmetrical-dimethyl arginine; spliceosomal snRNP biogenesis; protein amino acid methylation; transcription, DNA-dependent; rhythmic process; chromatin modification; negative regulation of transcription from RNA polymerase II promoter; endothelial cell activation; peptidyl-arginine methylation; negative regulation of cell differentiation; regulation of transcription, DNA-dependent; circadian regulation of gene expression; positive regulation of oligodendrocyte differentiation; peptidyl-arginine N-methylation