PRMT1 recombinant protein

PRMT1 protein

Cat. Num. AAA5304496

• Gene Names: PRMT1; ANM1; HCP1; IR1B4; HRMT1L2
• Purity: PRMT1 protein was purified using a a proprietary chromatographic technique.
• Synonyms: PRMT1; PRMT1 protein; PRMT 1 protein; Protein arginine N-methyltransferase 1 (EC 211-) (Histone-arginine N-methyltransferase PRMT1) (EC 211125) (Interferon receptor 1-bound protein 4)(EC 211-) (Histone-arginine N-methyltransferase PRMT1) (EC 211125) (Interferon receptor 1-bound protein 4) protein; PRMT 1; PRMT-1; PRMT-1 protein; PRMT1 recombinant protein

• Host: Human
• Purity/Purification: PRMT1 protein was purified using a a proprietary chromatographic technique.
• Form/Format: Supplied in liquid form in 40mM Tris-HCl pH 8.0, 100mM NaCl, 4mM MgCl2, 2mM DTT & 40% glycerol
• Sequence Length: 285

• Protein Type: Recombinant
• Biological Significance: PRMT1 methylate?s the guanidino nitrogens of arginyl residues present in a glycine and arginine-rich domain methylate?s SUPT5H. The PRMT1 protein functions as a histone methyltransferase specific for H4. PRMT1 is an essential factor in oncogenesis and is a potential novel therapeutic target in cancer. PRMT1-mediated methylation serves as a positive modulator of IR/IRS-1/PI3K pathway and glucose uptake in skeletal muscle cells. CAF1 is a new regulator of PRMT1-dependent arginine methylation. PRMT1 arginine-methylate?s MRE11 therefore it regulates the activity of MRE11-RAD50-NBS1 complex during the intra-S-phase DNA damage checkpoint response.
• Expression System: E Coli
• Preparation and Storage: Store at -80 degree C

Related Product Information for PRMT1 recombinant protein

Purified recombinant Human PRMT1 protein

Product Categories/Family for PRMT1 recombinant protein

Proteases; Inhibitors; & Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 333360913
• NCBI GeneID: 3276
• NCBI Accession #: NP_001193971.1
• NCBI GenBank Nucleotide #: NM_001207042.2
• Molecular Weight: 84.0 kDa
• NCBI Official Full Name: protein arginine N-methyltransferase 1 isoform 4
• NCBI Official Synonym Full Names: protein arginine methyltransferase 1
• NCBI Official Symbol: PRMT1
• NCBI Official Synonym Symbols: ANM1; HCP1; IR1B4; HRMT1L2
• NCBI Protein Information: protein arginine N-methyltransferase 1
• UniProt Protein Name: Protein arginine N-methyltransferase 1
• Protein Family: Protein arginine N-methyltransferase
• UniProt Gene Name: PRMT1
• UniProt Synonym Gene Names: HMT2; HRMT1L2; IR1B4
• UniProt Entry Name: ANM1_HUMAN

NCBI Description

This gene encodes a member of the protein arginine N-methyltransferase (PRMT) family. Post-translational modification of target proteins by PRMTs plays an important regulatory role in many biological processes, whereby PRMTs methylate arginine residues by transferring methyl groups from S-adenosyl-L-methionine to terminal guanidino nitrogen atoms. The encoded protein is a type I PRMT and is responsible for the majority of cellular arginine methylation activity. Increased expression of this gene may play a role in many types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 5. [provided by RefSeq, Dec 2011]

Uniprot Description

PRMT1: Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, PIAS1, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15 and EWS. Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. Together with dimethylated PIAS1, represses STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway. Homodimer and heterodimer with PRMT8. The dimer can then associate to form a homohexamer. Interacts with ILF3, BTG1, BTG2, SUPT5H and interferon-alpha/beta receptor 1. Interacts with NFATC2IP. Widely expressed. By BTG1, BTG2 and ILF3. Belongs to the protein arginine N-methyltransferase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Nuclear receptor co-regulator; Methyltransferase; Methyltransferase, protein arginine; EC 2.1.1.125

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: nucleoplasm; cytoplasm; nucleus; cytosol

Molecular Function: identical protein binding; methyltransferase activity; protein-arginine omega-N asymmetric methyltransferase activity; histone methyltransferase activity; protein binding; N-methyltransferase activity

Biological Process: histone methylation; establishment and/or maintenance of chromatin architecture; peptidyl-arginine methylation; cell surface receptor linked signal transduction; regulation of transcription, DNA-dependent; protein amino acid methylation; peptidyl-arginine methylation, to asymmetrical-dimethyl arginine; negative regulation of megakaryocyte differentiation; neurite development