Human protein arginine methyltransferase 1 ELISA Kit | PRMT1 elisa kit

Human protein arginine methyltransferase 1, PRMT1 ELISA Kit

Cat. Num. AAA9310012

• Gene Names: PRMT1; ANM1; HCP1; IR1B4; HRMT1L2
• Reactivity: Human
• Synonyms: protein arginine methyltransferase 1; Human protein arginine methyltransferase 1; PRMT1 ELISA Kit; Human protein arginine methyltransferase 1 (PRMT1) ELISA Kit; ANM1; HCP1; HRMT1L2; IR1B4; HMT1 (hnRNP methyltransferase; S. cerevisiae) -like 2; HMT1 hnRNP methyltransferase-like 2; interferon receptor 1-bound protein 4; protein arginine N-methyltransferase 1; PRMT1 elisa kit

• Reactivity: Human
• Specificity: No significant cross-reactivity or interference between this analyte and analogues is observed.

• Samples: Human body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances
• Assay Type: Sandwich
• Detection Range: 0.625ng/ml-20ng/ml
• Sensitivity: 0.1ng/ml
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100].
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for PRMT1 elisa kit

Background/Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only! This kit is intended to be used for determination the level of PRMT1 (hereafter termed "analyte") in undiluted original Human body fluids, tissue homogenates, secretions or feces samples. This kit is NOT suitable for assaying non-biological sources of substances.

NCBI and Uniprot Product Information

• NCBI GI #: 154759421
• NCBI GeneID: 3276
• NCBI Accession #: NP_001527.3
• NCBI GenBank Nucleotide #: NM_001536.5
• UniProt Accession #: Q99873; Q15529; Q2VP93; Q6LEU5; Q8WUW5; Q99872; Q99874; Q9NZ04; Q9NZ05; Q9NZ06; B4E3C3; G5E9B6
• Molecular Weight: 41,516 Da
• NCBI Official Full Name: protein arginine N-methyltransferase 1 isoform 1
• NCBI Official Synonym Full Names: protein arginine methyltransferase 1
• NCBI Official Symbol: PRMT1
• NCBI Official Synonym Symbols: ANM1; HCP1; IR1B4; HRMT1L2
• NCBI Protein Information: protein arginine N-methyltransferase 1; interferon receptor 1-bound protein 4; histone-arginine N-methyltransferase PRMT1; HMT1 (hnRNP methyltransferase, S. cerevisiae)-like 2; heterogeneous nuclear ribonucleoprotein methyltransferase 1-like 2
• UniProt Protein Name: Protein arginine N-methyltransferase 1
• Protein Family: Protein arginine N-methyltransferase
• UniProt Gene Name: PRMT1
• UniProt Synonym Gene Names: HMT2; HRMT1L2; IR1B4
• UniProt Entry Name: ANM1_HUMAN

NCBI Description

This gene encodes a member of the protein arginine N-methyltransferase (PRMT) family. Post-translational modification of target proteins by PRMTs plays an important regulatory role in many biological processes, whereby PRMTs methylate arginine residues by transferring methyl groups from S-adenosyl-L-methionine to terminal guanidino nitrogen atoms. The encoded protein is a type I PRMT and is responsible for the majority of cellular arginine methylation activity. Increased expression of this gene may play a role in many types of cancer. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene, and a pseudogene of this gene is located on the long arm of chromosome 5. [provided by RefSeq, Dec 2011]

Uniprot Description

PRMT1: Arginine methyltransferase that methylates (mono and asymmetric dimethylation) the guanidino nitrogens of arginyl residues present in proteins such as ESR1, histone H2, H3 and H4, PIAS1, HNRNPA1, HNRNPD, NFATC2IP, SUPT5H, TAF15 and EWS. Constitutes the main enzyme that mediates monomethylation and asymmetric dimethylation of histone H4 'Arg-4' (H4R3me1 and H4R3me2a, respectively), a specific tag for epigenetic transcriptional activation. Together with dimethylated PIAS1, represses STAT1 transcriptional activity, in the late phase of interferon gamma (IFN-gamma) signaling. May be involved in the regulation of TAF15 transcriptional activity, act as an activator of estrogen receptor (ER)-mediated transactivation, play a key role in neurite outgrowth and act as a negative regulator of megakaryocytic differentiation, by modulating p38 MAPK pathway. Homodimer and heterodimer with PRMT8. The dimer can then associate to form a homohexamer. Interacts with ILF3, BTG1, BTG2, SUPT5H and interferon-alpha/beta receptor 1. Interacts with NFATC2IP. Widely expressed. By BTG1, BTG2 and ILF3. Belongs to the protein arginine N-methyltransferase family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Methyltransferase, protein arginine; Nuclear receptor co-regulator; Methyltransferase; EC 2.1.1.125

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: nucleoplasm; cytoplasm; cytosol; nucleus

Molecular Function: identical protein binding; methyltransferase activity; histone methyltransferase activity; protein-arginine omega-N asymmetric methyltransferase activity; protein binding; N-methyltransferase activity

Biological Process: histone methylation; establishment and/or maintenance of chromatin architecture; peptidyl-arginine methylation; cell surface receptor linked signal transduction; regulation of transcription, DNA-dependent; protein amino acid methylation; peptidyl-arginine methylation, to asymmetrical-dimethyl arginine; negative regulation of megakaryocyte differentiation; neurite development