PKC nu / PRKD3 Recombinant Protein | PRKD3 recombinant protein

Recombinant Human PKC nu / PRKD3 Protein (GST tag)

Cat. Num. AAA2545102

• Gene Names: PRKD3; EPK2; PKD3; PRKCN; PKC-NU; nPKC-NU
• Purity: > 85 % as determined by SDS-PAGE
• Synonyms: PKC nu / PRKD3; Recombinant Human PKC nu / PRKD3 Protein (GST tag); EPK2; PKC-NU; PKD3; PRKCN; nPKC-NU; PRKD3 recombinant protein

• Host: Baculovirus-Insect Cells
• Purity/Purification: > 85 % as determined by SDS-PAGE
• Form/Format: Supplied as sterile 20mM Tris, 500mM NaCl, 10mM Reduced Glutathione, pH 7.4
• Sequence Length: 890

• Application Notes: The recombinant human PRKD3/GST chimera consists of 1114 amino acids and predicts a molecular mass of 126.7 kDa as estimated in SDS-PAGE under reducing conditions.
• Predicted N Terminal: Met
• Endotoxin: < 1.0 EU per mug of the protein as determined by the LAL method
• Preparation and Storage: Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

Related Product Information for PRKD3 recombinant protein

Background: Serine/threonine-protein kinase D3, also known as Protein kinase C nu type , Protein kinase EPK2, PRKD3, EPK2 and PRKCN, is a cytoplasm and membrane protein which belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and PKD subfamily. PRKD3 / PRKCN contains one PH domain, two phorbol-ester/DAG-type zinc fingers and one protein kinase domain. Protein kinase C (PKC) is a family of serine- and threonine-specific protein kinases that can be activated by calcium and the second messenger diacylglycerol. PKC family members phosphorylate a wide variety of protein targets and are known to be involved in diverse cellular signaling pathways. They also serve as major receptors for phorbol esters, a class of tumor promoters. Each member of the PKC family has a specific expression profile and is believed to play a distinct role. PRKD3 / PRKCN converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. It is involved in resistance to oxidative stress. PRKD3 / PRKCN is activated by DAG and phorbol esters. Phorbol-ester/DAG-type domains 1 and 2 bind both DAG and phorbol ester with high affinity and mediate translocation to the cell membrane. Autophosphorylation of Ser-735 and phosphorylation of Ser-731 by PKC relieves auto-inhibition by the PH domain. PRKD3 / PRKCN can be activated rapidly by the agonists of G protein-coupled receptors. It resides in both cytoplasm and nucleus, and its nuclear accumulation is found to be dramatically enhanced in response to its activation. PRKD3 / PRKCN can also be activated after B-cell antigen receptor (BCR) engagement, which requires intact phospholipase C gamma and the involvement of other PKC family members.

Description: A DNA sequence encoding the full length of human PRKD3 (NP_005804.1) (Met 1-Pro 890) was expressed with the GST tag at the N-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 5031689
• NCBI GeneID: 23683
• NCBI Accession #: NP_005804.1
• NCBI GenBank Nucleotide #: NM_005813.4
• UniProt Accession #: O94806; Q53TR7; Q8NEL8; D6W587
• Molecular Weight: 68,123 Da
• NCBI Official Full Name: serine/threonine-protein kinase D3
• NCBI Official Synonym Full Names: protein kinase D3
• NCBI Official Symbol: PRKD3
• NCBI Official Synonym Symbols: EPK2; PKD3; PRKCN; PKC-NU; nPKC-NU
• NCBI Protein Information: serine/threonine-protein kinase D3
• UniProt Protein Name: Serine/threonine-protein kinase D3
• Protein Family: Serine/threonine-protein kinase
• UniProt Gene Name: PRKD3
• UniProt Synonym Gene Names: EPK2; PRKCN

NCBI Description

This gene belongs to the multigene protein kinase D family of serine/threonine kinases, which bind diacylglycerol and phorbol esters. Members of this family are characterized by an N-terminal regulatory domain comprised of a tandem repeat of cysteine-rich zinc-finger motifs and a pleckstrin domain. The C-terminal region contains the catalytic domain and is distantly related to calcium-regulated kinases. Catalytic activity of this enzyme promotes its nuclear localization. This protein has been implicated in a variety of functions including negative regulation of human airway epithelial barrier formation, growth regulation of breast and prostate cancer cells, and vesicle trafficking. [provided by RefSeq, Jan 2015]

Uniprot Description

PRKD3: a CAMK kinase of the PKD family. An important component of signaling pathways downstream from novel PKC enzymes after B-cell receptor engagement. Novel isoforms regulate human keratinocyte differentiation

Protein type: CAMK group; EC 2.7.11.13; Kinase, protein; PKD family; Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 2p22.2

Cellular Component: cytoplasm; nucleoplasm; nucleus; plasma membrane

Molecular Function: kinase activity; protein binding

Biological Process: peptidyl-serine phosphorylation; protein amino acid phosphorylation; protein kinase C activation