PKC iota Blocking Peptide | PRKCI blocking peptide

PKC iota Antibody (N-term) Blocking Peptide

• Gene Names: PRKCI; PKCI; DXS1179E; nPKC-iota
• Synonyms: PKC iota; PKC iota Antibody (N-term) Blocking Peptide; Protein kinase C iota type; Atypical protein kinase C-lambda/iota; PRKC-lambda/iota; aPKC-lambda/iota; nPKC-iota; PRKCI; DXS1179E; PRKCI blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the N-term region of human PKC iota. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: The synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml deionized water for a final concentration of 1 mg/ml.
• Sequence Length: 596

• Cellular Location: Cytoplasm. Membrane. Endosome. Nucleus. Note: Transported into the endosome through interaction with SQSTM1/p62. After phosphorylation by SRC, transported into the nucleus through interaction with KPNB1. Colocalizes with CDK7 in the cytoplasm and nucleus. Transported to vesicular tubular clusters (VTCs) through interaction with RAB2A
• Tissue Location: Predominantly expressed in lung and brain, but also expressed at lower levels in many tissues including pancreatic islets. Highly expressed in non-small cell lung cancers.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for PRKCI blocking peptide

Calcium- and diacylglycerol-independent serine/ threonine-protein kinase that plays a general protective role against apoptotic stimuli, is involved in NF-kappa-B activation, cell survival, differentiation and polarity, and contributes to the regulation of microtubule dynamics in the early secretory pathway. Is necessary for BCR-ABL oncogene-mediated resistance to apoptotic drug in leukemia cells, protecting leukemia cells against drug-induced apoptosis. In cultured neurons, prevents amyloid beta protein-induced apoptosis by interrupting cell death process at a very early step. In glioblastoma cells, may function downstream of phosphatidylinositol 3-kinase (PI(3)K) and PDPK1 in the promotion of cell survival by phosphorylating and inhibiting the pro-apoptotic factor BAD. Can form a protein complex in non- small cell lung cancer (NSCLC) cells with PARD6A and ECT2 and regulate ECT2 oncogenic activity by phosphorylation, which in turn promotes transformed growth and invasion. In response to nerve growth factor (NGF), acts downstream of SRC to phosphorylate and activate IRAK1, allowing the subsequent activation of NF-kappa-B and neuronal cell survival. Functions in the organization of the apical domain in epithelial cells by phosphorylating EZR. This step is crucial for activation and normal distribution of EZR at the early stages of intestinal epithelial cell differentiation. Forms a protein complex with LLGL1 and PARD6B independently of PARD3 to regulate epithelial cell polarity. Plays a role in microtubule dynamics in the early secretory pathway through interaction with RAB2A and GAPDH and recruitment to vesicular tubular clusters (VTCs). In human coronary artery endothelial cells (HCAEC), is activated by saturated fatty acids and mediates lipid-induced apoptosis.

NCBI and Uniprot Product Information

• NCBI GI #: 239938658
• NCBI GeneID: 5584
• UniProt Accession #: P41743; Q8WW06; D3DNQ4
• Molecular Weight: 68,262 Da
• NCBI Official Full Name: Protein kinase C iota type
• NCBI Official Synonym Full Names: protein kinase C iota
• NCBI Official Symbol: PRKCI
• NCBI Official Synonym Symbols: PKCI; DXS1179E; nPKC-iota
• NCBI Protein Information: protein kinase C iota type
• UniProt Protein Name: Protein kinase C iota type
• Protein Family: Protein kinase
• UniProt Gene Name: PRKCI
• UniProt Synonym Gene Names: DXS1179E; PRKC-lambda/iota; aPKC-lambda/iota
• UniProt Entry Name: KPCI_HUMAN

NCBI Description

This gene encodes a member of the protein kinase C (PKC) family of serine/threonine protein kinases. The PKC family comprises at least eight members, which are differentially expressed and are involved in a wide variety of cellular processes. This protein kinase is calcium-independent and phospholipid-dependent. It is not activated by phorbolesters or diacylglycerol. This kinase can be recruited to vesicle tubular clusters (VTCs) by direct interaction with the small GTPase RAB2, where this kinase phosphorylates glyceraldehyde-3-phosphate dehydrogenase (GAPD/GAPDH) and plays a role in microtubule dynamics in the early secretory pathway. This kinase is found to be necessary for BCL-ABL-mediated resistance to drug-induced apoptosis and therefore protects leukemia cells against drug-induced apoptosis. There is a single exon pseudogene mapped on chromosome X. [provided by RefSeq, Jul 2008]

Uniprot Description

PKCI: an AGC protein kinase of the PKC family. A calcium-independent, phospholipid-dependent atypical protein kinase C. Is not activated by phorbolesters or diaglycerol. May play role in the secretory response to nutrients. Involved in cell polarization processes and the formation of epithelial tight junctions. Predominantly expressed in lung and brain, but also expressed at lower levels in many tissues including pancreatic islets.

Protein type: EC 2.7.11.13; Oncoprotein; Tumor suppressor; Motility/polarity/chemotaxis; Kinase, protein; Cell adhesion; Protein kinase, AGC; Cell development/differentiation; Protein kinase, Ser/Thr (non-receptor); AGC group; PKC family; Iota subfamily

Chromosomal Location of Human Ortholog: 3q26.3

Cellular Component: cytoplasm; cytosol; intercellular bridge; microtubule cytoskeleton; nucleus; plasma membrane

Molecular Function: ATP binding; phospholipid binding; protein binding; protein kinase activity; protein kinase C activity; protein serine/threonine kinase activity

Biological Process: activation of NF-kappaB transcription factor; cellular response to insulin stimulus; establishment and/or maintenance of epithelial cell polarity; intercellular junction assembly and maintenance; negative regulation of apoptosis; negative regulation of neuron apoptosis; peptidyl-serine phosphorylation; positive regulation of glucose import; protein amino acid phosphorylation; vesicle-mediated transport