Peroxiredoxin 6 (PRDX6) Recombinant Protein | PRDX6 recombinant protein

Recombinant Peroxiredoxin 6 (PRDX6)

Cat. Num. AAA2009150

• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: Peroxiredoxin 6 (PRDX6); Recombinant Peroxiredoxin 6 (PRDX6); PRDX6 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-MPGGLLLGDE APNFEANTTI GHIRFHDFLG DSWGILFSHP RDFTPVCTTE LGRAAKLAPE FAKRNVKLIA LSIDSVEDHF AWSKDINAYN GAAPTEKLPF PIIDDKDRDL AILLGMLDPA EKDEKGMPVT ARVVFIFGPD KKLKLSILYP ATTGRNFDEI LRVVDSLQLT ASNPVATPVD WKKGESVMVL PTLPEEEAKQ LFPKGVFTKE LPSGKKYLRY TPQP
• Sequence Length: 224

• Applicable Applications for PRDX6 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Rattus norvegicus (Rat)
• Expression System: Prokaryotic expression
• Residues: Met1~Pro224 (Accession # O35244) with N-terminal His-Tag
• Subcellular Location: Cytoplasm. Lysosome. Cytoplasmic vesicle
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Predicted Isoelectric Point: 6.1
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

SDS-Page

NCBI and Uniprot Product Information

• NCBI GI #: 16758348
• NCBI GeneID: 94167
• NCBI Accession #: NP_446028.1
• NCBI GenBank Nucleotide #: NM_053576.2
• UniProt Accession #: O35244
• Molecular Weight: 26.3kDa
• NCBI Official Full Name: peroxiredoxin-6
• NCBI Official Synonym Full Names: peroxiredoxin 6
• NCBI Official Symbol: Prdx6
• NCBI Protein Information: peroxiredoxin-6; NSGPx; aiPLA2; 1-Cys PRX; 1-Cys peroxiredoxin; antioxidant protein 2; thiol-specific antioxidant protein; non-selenium glutathione peroxidase; acidic calcium-independent phospholipase A2
• UniProt Protein Name: Peroxiredoxin-6
• Protein Family: Peroxiredoxin
• UniProt Gene Name: Prdx6
• UniProt Synonym Gene Names: Aipla2; Aop2; Tsa; 1-Cys PRX; aiPLA2; NSGPx
• UniProt Entry Name: PRDX6_RAT

NCBI Description

catalyzes the catabolism of hydrogen peroxide and organic hydroperoxides [RGD, Feb 2006]

Uniprot Description

PRDX6: Involved in redox regulation of the cell. Can reduce H(2)O(2) and short chain organic, fatty acid, and phospholipid hydroperoxides. May play a role in the regulation of phospholipid turnover as well as in protection against oxidative injury. Belongs to the AhpC/TSA family. Rehydrin subfamily.

Protein type: Amino Acid Metabolism - phenylalanine; Oxidoreductase; EC 1.11.1.9; Phospholipase; Energy Metabolism - methane; EC 1.11.1.15

Cellular Component: extracellular space; membrane; mitochondrion; lysosome; cytoplasmic membrane-bound vesicle; cytoplasm; cytosol

Molecular Function: phospholipase A2 activity; protein binding; peroxidase activity; peroxiredoxin activity; glutathione peroxidase activity

Biological Process: response to reactive oxygen species; hydrogen peroxide catabolic process; phospholipid catabolic process; bleb formation; response to oxidative stress