PR Domain Containing Protein 1 (PRDM1) Recombinant Protein | PRDM1 recombinant protein

Recombinant PR Domain Containing Protein 1 (PRDM1)

Cat. Num. AAA2009817

• Gene Names: PRDM1; BLIMP1; PRDI-BF1
• Applications: SDS-Page, Western Blot, ELISA, Immunoprecipitation
• Purity: > 95%
• Synonyms: PR Domain Containing Protein 1 (PRDM1); Recombinant PR Domain Containing Protein 1 (PRDM1); PRDM1 recombinant protein

• Host: E Coli
• Purity/Purification: > 95%
• Form/Format: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.
• Sequence: The target protein is fused with N-terminal His-Tag, its sequence is listed below.; MGHHHHHHSGSEF-LPRNLLFK YATNSEEVIG VMSKEYIPKG TRFGPLIGEI YTNDTVPKNA NRKYFWRIYS RGELHHFIDG FNEEKSNWMR YVNPAHSPRE QNLAACQNGM NIYFYTIKPI PANQELLVWY CRD
• Sequence Length: 825

• Applicable Applications for PRDM1 recombinant protein: SDS-PAGE, Western Blot (WB), ELISA (EIA), Immunoprecipitation (IP)
• Organism: Homo sapiens (Human)
• Expression System: Prokaryotic expression
• Residues: Leu83~Asp203 (Accession # O75626) with N-terminal His-Tag
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.
• Preparation and Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 degree C for one month. Aliquot and store at -80 degree C for 12 months.; Stability Test: The thermal stability is described by the loss rate of the targetprotein. The loss rate was determined by accelerated thermal degradation test,that is, incubate the protein at 37 degree C for 48h, and no obvious degradation andprecipitation were observed. (Referring from China Biological Products Standard,which was calculated by the Arrhenius equation.) The loss of this protein is lessthan 5% within the expiration date under appropriate storage condition.

Sequence Information

SDS-Page

Testing Data

NCBI and Uniprot Product Information

• NCBI GI #: 172072684
• NCBI GeneID: 639
• NCBI Accession #: NP_001189.2
• NCBI GenBank Nucleotide #: NM_001198.3
• UniProt Accession #: O75626; Q86WM7; B2REA6; E1P5E0
• Molecular Weight: 15.8kDa
• NCBI Official Full Name: PR domain zinc finger protein 1 isoform 1
• NCBI Official Synonym Full Names: PR domain containing 1, with ZNF domain
• NCBI Official Symbol: PRDM1
• NCBI Official Synonym Symbols: BLIMP1; PRDI-BF1
• NCBI Protein Information: PR domain zinc finger protein 1; BLIMP-1; PRDI-binding factor-1; B-lymphocyte-induced maturation protein 1; beta-interferon gene positive-regulatory domain I binding factor
• UniProt Protein Name: PR domain zinc finger protein 1
• Protein Family: PR domain zinc finger protein
• UniProt Gene Name: PRDM1
• UniProt Synonym Gene Names: BLIMP1; PRDI-BF1
• UniProt Entry Name: PRDM1_HUMAN

NCBI Description

This gene encodes a protein that acts as a repressor of beta-interferon gene expression. The protein binds specifically to the PRDI (positive regulatory domain I element) of the beta-IFN gene promoter. Transcription of this gene increases upon virus induction. Two alternatively spliced transcript variants that encode different isoforms have been reported. [provided by RefSeq, Jul 2008]

Uniprot Description

PRDM1: a transcriptional repressor that binds specifically to the PRDI element in the promoter of the beta-interferon gene and can inhibit virus-mediated IFN-¿ production. Recruits chromatin-modifying enzymes including histone deacetylases and methyltransferases. Drives the maturation of B-lymphocytes into Ig secreting cells. Interacts with PRMT5. Expression of Blimp-1 is sufficient to drive terminal differentiation of BCL1 lymphoma cells into antibody secreting plasma cells, increasing the expression of the cell surface marker Syndecan-1. In the B-cell lineage, Blimp-1 is specifically expressed in antibody-secreting cells including activated B and plasma cells. In addition, Blimp-1 has been found during macrophage differentiation and in a subset of T-cells suggesting that it may play a wider role in homeostasis and differentiation. Target genes of Blimp-1 transcriptional repression with potential roles in differentiation include c-Myc, CIITA, Pax5, Spi-B, and Id3.Three isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription regulation; C2H2-type zinc finger protein; Methyltransferase, protein lysine, predicted; DNA-binding; Transcription, coactivator/corepressor

Chromosomal Location of Human Ortholog: 6q21

Cellular Component: cytoplasm; nucleus

Molecular Function: methyltransferase activity; protein binding; metal ion binding; histone deacetylase binding; transcription factor activity

Biological Process: methylation; negative regulation of lipopolysaccharide-mediated signaling pathway; eye photoreceptor cell development; cell fate commitment; transcription, DNA-dependent; positive regulation of B cell differentiation; negative regulation of transcription from RNA polymerase II promoter; negative regulation of B cell proliferation; germ cell development; maternal placenta development; post-embryonic development