Rabbit anti-Human PPP1R16B Polyclonal Antibody | anti-PPP1R16B antibody

PPP1R16B Antibody (Center)

Cat. Num. AAA289069

• Gene Names: PPP1R16B; TIMAP; ANKRD4
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Synonyms: PPP1R16B, Antibody; PPP1R16B Antibody (Center); Protein phosphatase 1 regulatory inhibitor subunit 16B; Ankyrin repeat domain-containing protein 4; CAAX box protein TIMAP; TGF-beta-inhibited membrane-associated protein; hTIMAP; PPP1R16B; ANKRD4; KIAA0823; anti-PPP1R16B antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: This PPP1R16B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 372-399 amino acids from the Central region of human PPP1R16B.
• Purity/Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Sequence Positions: 372-399
• Sequence Length: 525

• Applicable Applications for anti-PPP1R16B antibody: ELISA, WB (Western Blot)
• Function: Regulator of protein phosphatase 1 (PP1) that acts as a positive regulator of pulmonary endothelial cell (EC) barrier function (PubMed:18586956). Involved in the regulation of the PI3K/AKT signaling pathway, angiogenesis and endothelial cell proliferation (PubMed:25007873). Regulates angiogenesis and endothelial cell proliferation through the control of ECE1 dephosphorylation, trafficking and activity (By similarity). Protects the endothelial barrier from lipopolysaccharide (LPS)-induced vascular leakage (By similarity). Involved in the regulation of endothelial cell filopodia extension (By similarity). May be a downstream target for TGF-beta1 signaling cascade in endothelial cells (PubMed:16263087, PubMed:18586956). Involved in PKA-mediated moesin dephosphorylation which is important in EC barrier protection against thrombin stimulation (PubMed:18586956). Promotes the interaction of PPP1CA with RPSA/LAMR1 and in turn facilitates the dephosphorylation of RPSA/LAMR1 (PubMed:16263087). Involved in the dephosphorylation of EEF1A1 (PubMed:26497934).
• Cellular Location: Cell membrane. Cell membrane; Lipid-anchor. Nucleus. Cell projection. Note=Colocalizes with RPSA/LAMR1 in the cell membrane (PubMed:16263087). Localizes to the perinuclear region (By similarity). Colocalizes with PTEN at the tip of EC projections (PubMed:25007873). {ECO:0000250|UniProtKB:Q95N27, ECO:0000269|PubMed:16263087, ECO:0000269|PubMed:25007873}
• Preparation and Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

WB (Western Blot)

(PPP1R16B Antibody (Center) western blot analysis in HepG2 cell line lysates (35ug/lane).This demonstrates the PPP1R16B antibody detected the PPP1R16B protein (arrow).)

Related Product Information for anti-PPP1R16B antibody

The protein encoded by this gene is membrane-associated and contains five ankyrin repeats, a protein phosphatase-1-interacting domain, and a carboxy-terminal CAAX box domain. Synthesis of the encoded protein is inhibited by transforming growth factor beta-1. The protein may bind to the membrane through its CAAX box domain and may act as a signaling molecule through interaction with protein phosphatase-1.Alternatively spliced transcript variants encoding different isoforms have been identified in this gene.

Product Categories/Family for anti-PPP1R16B antibody

Signal Transduction

References

Csortos, C., et al. Am. J. Physiol. Lung Cell Mol. Physiol. 295 (3), L440-L450 (2008) :
Kim, K., et al. Biochem. Biophys. Res. Commun. 338(3):1327-1334(2005)
Homma, K., et al. J. Mol. Biol. 343(5):1207-1220(2004)
Cao, W., et al. Am. J. Physiol., Cell Physiol. 283 (1), C327-C337 (2002) :
Deloukas, P., et al. Nature 414(6866):865-871(2001)

NCBI and Uniprot Product Information

• NCBI GI #: 289629249
• NCBI GeneID: 26051
• NCBI Accession #: NP_001166206.1; NP_056383.1
• NCBI GenBank Nucleotide #: NM_001172735.1
• UniProt Accession #: Q96T49; O94912; Q5W9G4; Q9NQG4; A2RRR6; E9PFS8
• Molecular Weight: Calculated MW: 63551
• NCBI Official Full Name: protein phosphatase 1 regulatory inhibitor subunit 16B isoform 2
• NCBI Official Synonym Full Names: protein phosphatase 1, regulatory subunit 16B
• NCBI Official Symbol: PPP1R16B
• NCBI Official Synonym Symbols: TIMAP; ANKRD4
• NCBI Protein Information: protein phosphatase 1 regulatory inhibitor subunit 16B
• UniProt Protein Name: Protein phosphatase 1 regulatory inhibitor subunit 16B
• UniProt Gene Name: PPP1R16B
• UniProt Synonym Gene Names: ANKRD4; KIAA0823; hTIMAP
• UniProt Entry Name: PP16B_HUMAN