SDS-PAGE
PP2B recombinant protein
PP2B recombinant protein
Gene Names
PPP3CA; CALN; CCN1; CNA1; CALNA; PPP2B; CALNA1
Applications
Western Blot
Synonyms
PP2B; PP2B recombinant protein; PPP2B; PPP3CA; CALN; CCN1; CNA1; CALNA; CALNA1; PP2B Protein
Host
Sf9 insect cells
Form/Format
50mM Tris-HCl, pH 7.5, 150mM NaCl, 10mM glutathione, 0.1mM EDTA, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.
Sequence Length
4646
Applicable Applications for PP2B recombinant protein
Western Blot (WB)
Type
Recombinant Fusion Protein
Species
Human
Tag Information
GST tag
Expression System
Sf9 insect cells using baculovirus
Source Note
Recombinant full-length human PP2B was expressed by baculovirus in Sf9 insect cells
Preparation and Storage
Store product at -70 degree C. For optimal storage, aliquot target into smaller quantities after centrifugation and store at recommended temperature. For most favorable performance, avoid repeated handling and multiple freeze/thaw cycles.
SDS-PAGE
SDS-PAGE
Related Product Information for PP2B recombinant protein
Recombinant full-length human PP2B was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag.
Scientific Background: PP2B is a key regulator of the anaphase-promoting factor which is an excellent substrate for protein phosphatase. PP2B is involved in bovine ocular tissues and is postulated to be involved in the immunologic privilege of the cornea, in retinal signal transduction, and in the toxic effects of immunosuppressants on the eye. PP2B acts as an inhibitory constraint on I-LTP that is relieved by PKA, and that this inhibitory constraint acts as a gate to regulate the synaptic induction of L-LTP (1). PP2B plays a role in the transition from short- to long-term memory and also regulates bone formation through an effect on osteoblast differentiation (2).
Scientific Background: PP2B is a key regulator of the anaphase-promoting factor which is an excellent substrate for protein phosphatase. PP2B is involved in bovine ocular tissues and is postulated to be involved in the immunologic privilege of the cornea, in retinal signal transduction, and in the toxic effects of immunosuppressants on the eye. PP2B acts as an inhibitory constraint on I-LTP that is relieved by PKA, and that this inhibitory constraint acts as a gate to regulate the synaptic induction of L-LTP (1). PP2B plays a role in the transition from short- to long-term memory and also regulates bone formation through an effect on osteoblast differentiation (2).
Product Categories/Family for PP2B recombinant protein
References
1. Winder. et.al: Genetic and pharmacological evidence for a novel, intermediate phase of long-term potentiation suppressed by calcineurin. Cell 92: 25-37, 1998.
2. Sun, L. et.al: Calcineurin regulates bone formation by the osteoblast. Proc. Nat. Acad. Sci. 102: 17130-17135, 2005.
2. Sun, L. et.al: Calcineurin regulates bone formation by the osteoblast. Proc. Nat. Acad. Sci. 102: 17130-17135, 2005.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
~83 kDa
NCBI Official Full Name
Homo sapiens protein phosphatase 3, catalytic subunit, alpha isozyme (PPP3CA), transcript variant 2, mRNA
NCBI Official Synonym Full Names
protein phosphatase 3, catalytic subunit, alpha isozyme
NCBI Official Symbol
PPP3CA
NCBI Official Synonym Symbols
CALN; CCN1; CNA1; CALNA; PPP2B; CALNA1
NCBI Protein Information
serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform; calcineurin A alpha; CAM-PRP catalytic subunit; calmodulin-dependent calcineurin A subunit alpha isoform; protein phosphatase 2B, catalytic subunit, alpha isoform; protein phosphatase 3 (formerly 2B), catalytic subunit, alpha isoform
UniProt Protein Name
Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform
UniProt Gene Name
PPP3CA
UniProt Synonym Gene Names
CALNA; CNA
UniProt Entry Name
PP2BA_HUMAN
Uniprot Description
Function: Calcium-dependent, calmodulin-stimulated protein phosphatase. Many of the substrates contain a PxIxIT motif. This subunit may have a role in the calmodulin activation of calcineurin. Dephosphorylates DNM1L, HSPB1 and SSH1. Ref.12 Ref.13 Ref.16
Catalytic activity: A phosphoprotein + H2O = a protein + phosphate.
Cofactor: Binds 1 Fe3+ ion per subunit.Binds 1 zinc ion per subunit.
Subunit structure: Interacts with CHP1 and CHP2
By similarity. Composed of two components (A and B), the A component is the catalytic subunit and the B component confers calcium sensitivity. Interacts with CRTC2, MYOZ1, MYOZ2 and MYOZ3. Interacts with DNM1L; the interaction dephosphorylates DNM1L and regulates its translocation to mitochondria. Interacts with CMYA5; this interaction represses calcineurin activity in muscle
By similarity. Ref.9 Ref.10 Ref.11 Ref.13
Subcellular location: Nucleus
By similarity. Note: Colocalizes with ACTN1 and MYOZ2 at the Z line in heart and skeletal muscle
By similarity.
Sequence similarities: Belongs to the PPP phosphatase family. PP-2B subfamily.
Research Articles on PP2B
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Product Notes
The PP2B ppp3ca (Catalog #AAA515423) is a Recombinant Protein produced from Sf9 insect cells and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is GST tag!!Expression System||Sf9 insect cells using baculovirus!!Source Note||Recombinant full-length human PP2B was expressed by baculovirus in Sf9 insect cells. AAA Biotech's PP2B can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the PP2B ppp3ca for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "PP2B, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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