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SDS-PAGE

Phosphoenolpyruvate carboxylase Recombinant Protein | ppc recombinant protein

Recombinant E Coli Phosphoenolpyruvate carboxylase

Gene Names
ppc; asp; ECK3947; glu; JW3928
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Synonyms
Phosphoenolpyruvate carboxylase; Recombinant E Coli Phosphoenolpyruvate carboxylase; ppc recombinant protein
Ordering
For Research Use Only!
Host
E Coli
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE.
Form/Format
Liquid containing glycerol
Sequence Positions
5-883aa; Partial
Sequence
YSALRSNVSMLGKVLGETIKDALGEHILERVETIRKLSKSSRAGNDANRQELLTTLQNLSNDELLPVARAFSQFLNLANTAEQYHSISPKGEAASNPEVIARTLRKLKNQPELSEDTIKKAVESLSLELVLTAHPTEITRRTLIHKMVEVNACLKQLDNKDIADYEHNQLMRRLRQLIAQSWHTDEIRKLRPSPVDEAKWGFAVVENSLWQGVPNYLRELNEQLEENLGYKLPVEFVPVRFTSWMGGDRDGNPNVTADITRHVLLLSRWKATDLFLKDIQVLVSELSMVEATPELLALVGEEGAAEPYRYLMKNLRSRLMATQAWLEARLKGEELPKPEGLLTQNEELWEPLYACYQSLQACGMGIIANGDLLDTLRRVKCFGVPLVRIDIRQESTRHTEALGELTRYLGIGDYESWSEADKQAFLIRELNSKRPLLPRNWQPSAETREVLDTCQVIAEAPQGSIAAYVISMAKTPSDVLAVHLLLKEAGIGFAMPVAPLFETLDDLNNANDVMTQLLNIDWYRGLIQGKQMVMIGYSDSAKDAGVMAASWAQYQAQDALIKTCEKAGIELTLFHGRGGSIGRGGAPAHAALLSQPPGSLKGGLRVTEQGEMIRFKYGLPEITVSSLSLYTGAILEANLLPPPEPKESWRRIMDELSVISCDVYRGYVRENKDFVPYFRSATPEQELGKLPLGSRPAKRRPTGGVESLRAIPWIFAWTQNRLMLPAWLGAGTALQKVVEDGKQSELEAMCRDWPFFSTRLGMLEMVFAKADLWLAEYYDQRLVDKALWPLGKELRNLQEEDIKVVLAIANDSHLMADLPWIAESIQLRNIYTDPLNVLQAELLHRSRQAEKEGQEPDPRVEQALMVTIAGIAAGMRNTG
Sequence Length
883
Preparation and Storage
Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C.

SDS-PAGE

SDS-PAGE
Related Product Information for ppc recombinant protein
Forms oxaloacetate, a four-carbon dicarboxylic acid source for the tricarboxylic acid cycle.
References
The primary structure of phosphoenolpyruvate carboxylase of Escherichia coli. Nucleotide sequence of the ppc gene and deduced amino acid sequence.Fujita N., Miwa T., Ishijima S., Izui K., Katsuki H.J. Biochem. 95:909-916(1984) Analysis of the Escherichia coli genome. IV. DNA sequence of the region from 89.2 to 92.8 minutes.Blattner F.R., Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L.Nucleic Acids Res. 21:5408-5417(1993) The complete genome sequence of Escherichia coli K-12.Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.Science 277:1453-1462(1997) Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110.Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.Mol. Syst. Biol. 2:E1-E5(2006) Structural and biochemical characterization of the Escherichia coli argE gene product.Meinnel T., Schmitt E., Mechulam Y., Blanquet S.J. Bacteriol. 174:2323-2331(1992) Promoter analysis of the phosphoenolpyruvate carboxylase gene of Escherichia coli.Izui K., Miwa T., Kajitani M., Fujita N., Sabe H., Ishihama I., Katsuki H.Nucleic Acids Res. 13:59-71(1985) Site-directed mutagenesis of phosphoenolpyruvate carboxylase from E. coli the role of His579 in the catalytic and regulatory functions.Terada K., Murata T., Izui K.J. Biochem. 109:49-54(1991) Site-directed mutagenesis of the conserved histidine residue of phosphoenolpyruvate carboxylase. His138 is essential for the second partial reaction.Terada K., Izui K.Eur. J. Biochem. 202:797-803(1991) Catalytic role of an arginine residue in the highly conserved and unique sequence of phosphoenolpyruvate carboxylase.Yano M., Terada K., Umiji K., Izui K.J. Biochem. 117:1196-1200(1995) First crystallization of a phosphoenolpyruvate carboxylase from Escherichia coli.Inoue M., Hayashi M., Sugimoto M., Harada S., Kai Y., Kasai N., Terada K., Izui K.J. Mol. Biol. 208:509-510(1989) Escherichia coli proteome analysis using the gene-protein database.VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.Electrophoresis 18:1243-1251(1997) Three-dimensional structure of phosphoenolpyruvate carboxylase a proposed mechanism for allosteric inhibition.Kai Y., Matsumura H., Inoue T., Terada K., Nagara Y., Yoshinaga T., Kihara A., Tsumura K., Izui K.Proc. Natl. Acad. Sci. U.S.A. 96:823-828(1999)

NCBI and Uniprot Product Information

NCBI GeneID
UniProt Accession #
Molecular Weight
102.6 kDa
NCBI Official Symbol
ppc
NCBI Official Synonym Symbols
asp; ECK3947; glu; JW3928
NCBI Protein Information
phosphoenolpyruvate carboxylase
UniProt Protein Name
Phosphoenolpyruvate carboxylase
UniProt Gene Name
ppc
UniProt Synonym Gene Names
glu; PEPC; PEPCase
UniProt Entry Name
CAPP_ECOLI

NCBI Description

Mutant has reduced growth rate with little acetate excreation, decrease glucose consumption and a decreased carbon dioxide evolution rate. [More information is available at EcoGene: EG10756]. Mutants in the ppc gene have been isolated and studied . [More information is available at EcoCyc: EG10756].

Uniprot Description

Forms oxaloacetate, a four-carbon dicarboxylic acid source for the tricarboxylic acid cycle.

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Product Notes

The ppc ppc (Catalog #AAA1265338) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 5-883aa; Partial. The amino acid sequence is listed below: YSALRSNVSM LGKVLGETIK DALGEHILER VETIRKLSKS SRAGNDANRQ ELLTTLQNLS NDELLPVARA FSQFLNLANT AEQYHSISPK GEAASNPEVI ARTLRKLKNQ PELSEDTIKK AVESLSLELV LTAHPTEITR RTLIHKMVEV NACLKQLDNK DIADYEHNQL MRRLRQLIAQ SWHTDEIRKL RPSPVDEAKW GFAVVENSLW QGVPNYLREL NEQLEENLGY KLPVEFVPVR FTSWMGGDRD GNPNVTADIT RHVLLLSRWK ATDLFLKDIQ VLVSELSMVE ATPELLALVG EEGAAEPYRY LMKNLRSRLM ATQAWLEARL KGEELPKPEG LLTQNEELWE PLYACYQSLQ ACGMGIIANG DLLDTLRRVK CFGVPLVRID IRQESTRHTE ALGELTRYLG IGDYESWSEA DKQAFLIREL NSKRPLLPRN WQPSAETREV LDTCQVIAEA PQGSIAAYVI SMAKTPSDVL AVHLLLKEAG IGFAMPVAPL FETLDDLNNA NDVMTQLLNI DWYRGLIQGK QMVMIGYSDS AKDAGVMAAS WAQYQAQDAL IKTCEKAGIE LTLFHGRGGS IGRGGAPAHA ALLSQPPGSL KGGLRVTEQG EMIRFKYGLP EITVSSLSLY TGAILEANLL PPPEPKESWR RIMDELSVIS CDVYRGYVRE NKDFVPYFRS ATPEQELGKL PLGSRPAKRR PTGGVESLRA IPWIFAWTQN RLMLPAWLGA GTALQKVVED GKQSELEAMC RDWPFFSTRL GMLEMVFAKA DLWLAEYYDQ RLVDKALWPL GKELRNLQEE DIKVVLAIAN DSHLMADLPW IAESIQLRNI YTDPLNVLQA ELLHRSRQAE KEGQEPDPRV EQALMVTIAG IAAGMRNTG. It is sometimes possible for the material contained within the vial of "Phosphoenolpyruvate carboxylase, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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