Typical Testing Data/Standard Curve (for reference only)
Guinea Pig Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha ELISA Kit | PPARgC1a elisa kit
Guinea Pig Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha ELISA Kit
Gene Names
Ppargc1a; Ppargc1
Reactivity
Guinea Pig
Synonyms
Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha; Guinea Pig Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha ELISA Kit; PPARgC1a elisa kit
Reactivity
Guinea Pig
Specificity
This assay has high sensitivity and excellent specificity for detection of PPARG1A. No significant cross-reactivity or interference between PPARG1A and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PPARG1A and all the analogues, therefore, cross reaction may still exist in some cases.
Sequence Length
796
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
1.0 pg/mL
Reactivity Note
No significant cross-reactivity or interference between this antigen and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection, therefore, cross reaction may still exist in some cases.
Preparation and Storage
Store at 2-8 degree C.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for PPARgC1a elisa kit
Intended Uses: This PPARG1A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Guinea pig PPARG1A. This ELISA kit for research use only!
Principle of the Assay: PPARG1A ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-PPARG1A antibody and an PPARG1A-HRP conjugate. The assay sample and buffer are incubated together with PPARG1A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PPARG1A concentration since PPARG1A from samples and PPARG1A-HRP conjugate compete for the anti-PPARG1A antibody binding site. Since the number of sites is limited, as more sites are occupied by PPARG1A from the sample, fewer sites are left to bind PPARG1A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PPARG1A concentration in each sample is interpolated from this standard curve.
Principle of the Assay: PPARG1A ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-PPARG1A antibody and an PPARG1A-HRP conjugate. The assay sample and buffer are incubated together with PPARG1A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PPARG1A concentration since PPARG1A from samples and PPARG1A-HRP conjugate compete for the anti-PPARG1A antibody binding site. Since the number of sites is limited, as more sites are occupied by PPARG1A from the sample, fewer sites are left to bind PPARG1A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PPARG1A concentration in each sample is interpolated from this standard curve.
Product Categories/Family for PPARgC1a elisa kit
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
90,622 Da
NCBI Official Full Name
peroxisome proliferator-activated receptor gamma coactivator 1-alpha
NCBI Official Synonym Full Names
PPARG coactivator 1 alpha
NCBI Official Symbol
Ppargc1a
NCBI Official Synonym Symbols
Ppargc1
NCBI Protein Information
peroxisome proliferator-activated receptor gamma coactivator 1-alpha
UniProt Protein Name
Peroxisome proliferator-activated receptor gamma coactivator 1-alpha
Protein Family
UniProt Gene Name
Ppargc1a
UniProt Synonym Gene Names
Pgc1; Pgc1a; Ppargc1; PGC-1-alpha; PPAR-gamma coactivator 1-alpha; PPARGC-1-alpha
NCBI Description
nuclear receptor coactivator; may mediate glucose homeostasis, and lipid homeostasis [RGD, Feb 2006]
Uniprot Description
Transcriptional coactivator for steroid receptors and nuclear receptors. Greatly increases the transcriptional activity of PPARG and thyroid hormone receptor on the uncoupling protein promoter. Can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis. Plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. Induces the expression of PERM1 in the skeletal muscle in an ESRRA-dependent manner. Also involved in the integration of the circadian rhythms and energy metabolism. Required for oscillatory expression of clock genes, such as ARNTL/BMAL1 and NR1D1, through the coactivation of RORA and RORC, and metabolic genes, such as PDK4 and PEPCK ().
Research Articles on PPARgC1a
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Product Notes
The Guinea Pig PPARgC1a ppargc1a (Catalog #AAA7258086) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7258086 ELISA Kit recognizes Guinea Pig PPARgC1a. It is sometimes possible for the material contained within the vial of "Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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