OCT4 (OCT3) (E125) Blocking Peptide | POU5F1 blocking peptide

OCT4 (OCT3) Antibody (E125) Blocking peptide

• Gene Names: POU5F1; OCT3; OCT4; OTF3; OTF4; OTF-3; Oct-3; Oct-4
• Synonyms: OCT4 (OCT3) (E125); OCT4 (OCT3) Antibody (E125) Blocking peptide; POU domain; class 5; transcription factor 1; Octamer-binding protein 3; Oct-3; Octamer-binding protein 4; Oct-4; Octamer-binding transcription factor 3; OTF-3; POU5F1; OCT3; OCT4; OTF3; POU5F1 blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the E125 region of human OCT3/4. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 360

• Cellular Location: Cytoplasm. Nucleus. Note: Expressed in a diffuse and slightly punctuate pattern
• Tissue Location: Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues.
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for POU5F1 blocking peptide

Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency.

NCBI and Uniprot Product Information

• NCBI GI #: 400659
• NCBI GeneID: 5460
• UniProt Accession #: Q01860; P31359; Q15167; Q15168; Q16422; Q5STF3; Q5STF4; A6NCS1; A6NLL8; D2IYK4
• Molecular Weight: 30,085 Da
• NCBI Official Full Name: POU domain, class 5, transcription factor 1
• NCBI Official Synonym Full Names: POU class 5 homeobox 1
• NCBI Official Symbol: POU5F1
• NCBI Official Synonym Symbols: OCT3; OCT4; OTF3; OTF4; OTF-3; Oct-3; Oct-4
• NCBI Protein Information: POU domain, class 5, transcription factor 1
• UniProt Protein Name: POU domain, class 5, transcription factor 1
• Protein Family: POU domain, class 5, transcription factor
• UniProt Gene Name: POU5F1
• UniProt Synonym Gene Names: OCT3; OCT4; OTF3; Oct-3; Oct-4; OTF-3
• UniProt Entry Name: PO5F1_HUMAN

NCBI Description

This gene encodes a transcription factor containing a POU homeodomain that plays a key role in embryonic development and stem cell pluripotency. Aberrant expression of this gene in adult tissues is associated with tumorigenesis. This gene can participate in a translocation with the Ewing's sarcoma gene on chromosome 21, which also leads to tumor formation. Alternative splicing, as well as usage of alternative AUG and non-AUG translation initiation codons, results in multiple isoforms. One of the AUG start codons is polymorphic in human populations. Related pseudogenes have been identified on chromosomes 1, 3, 8, 10, and 12. [provided by RefSeq, Oct 2013]

Uniprot Description

Oct4: Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3'). Forms a trimeric complex with SOX2 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206. Critical for early embryogenesis and for embryonic stem cell pluripotency. Interacts with UBE2I and ZSCAN10. Interacts with PKM2. Interacts with WWP2. Transcriptional activity is positively regulated by PKM2. Expressed in developing brain. Highest levels found in specific cell layers of the cortex, the olfactory bulb, the hippocampus and the cerebellum. Low levels of expression in adult tissues. Belongs to the POU transcription factor family. Class- 5 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Transcription factor; DNA-binding

Chromosomal Location of Human Ortholog: 6p21.31

Cellular Component: cytoplasm; cytosol; nucleoplasm; nucleus

Molecular Function: DNA binding; miRNA binding; protein binding; sequence-specific DNA binding; transcription factor activity; ubiquitin protein ligase binding

Biological Process: anatomical structure morphogenesis; blastocyst development; endodermal cell fate specification; mRNA transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; positive regulation of transcription from RNA polymerase II promoter; regulation of asymmetric cell division; regulation of gene expression; regulation of transcription, DNA-dependent; response to wounding; somatic stem cell maintenance; transcription from RNA polymerase II promoter