ErbB 4 positive control

ErbB 4 Positive Control

Cat. Num. AAA542839

• Gene Names: ERBB4; HER4; ALS19; p180erbB4
• Applications: Western Blot
• Synonyms: ErbB 4; ErbB 4 Positive Control; 4ICD; ALS19; E4ICD; EC 2.7.10.1; ERBB4; HER4; Mer4; MGC138404; Oncogene ERBB4; p180erbB4; Proto-oncogene-like protein c-ErbB-4; Receptor tyrosine protein kinase erbB 4; s80HER4; Tyrosine kinase type cell surface receptor HER4 antibodyv erb a avian erythroblastic leukemia viral oncogene homolog like 4 antibody; ErbB 4 positive control

• Form/Format: Western Blot Positive Control
• Sequence Length: 1308

• Applicable Applications for ErbB 4 positive control: Western Blot (WB)
• Application Notes: Western Blot: 1:500
• Immunogen: Purified Protein
• Expression: Expressed at highest levels in brain, heart, kidney, in addition to skeletal muscle, parathyroid, cerebellum, pituitary, spleen, testis and breast. Lower levels in thymus, lung, salivary gland, and pancreas. Isoform JM-A CYT-1 and isoform JM-B CYT-1 are expressed in cerebellum, but only the isoform JM-B is expressed in the heart.
• Molecular Function: ATP binding; epidermal growth factor receptor binding; protein homodimerization activity; receptor signaling protein tyrosine kinase activity; transcription regulatory region DNA binding; transmembrane receptor protein tyrosine kinase activity
• Structure: Belongs to the protein kinase superfamily. Tyr protein kinase family. EGF receptor subfamily.; Contains 1 protein kinase domain.
• Subcellular Location: Cell membrane; Single-pass type I membrane protein. ERBB4 intracellular domain: Nucleus; Mitochondrion
• Preparation and Storage: -20 degree C for long term storage

Related Product Information for ErbB 4 positive control

Western Blot Positive Control Receptor tyrosine-protein kinase erB-4 Antibody
Tyrosine-protein kinase that plays an essential role as cell surface receptor for neuregulins and EGF family members and regulates development of the heart, the central nervous system and the mammary gland, gene transcription, cell proliferation, differentiation, migration and apoptosis. Required for normal cardiac muscle differentiation during embryonic development, and for postnatal cardiomyocyte proliferation. Required for normal development of the embryonic central nervous system, especially for normal neural crest cell migration and normal axon guidance. Required for mammary gland differentiation, induction of milk proteins and lactation. Acts as cell-surface receptor for the neuregulins NRG1, NRG2, NRG3 and NRG4 and the EGF family members BTC, EREG and HBEGF. Ligand binding triggers receptor dimerization and autophosphorylation at specific tyrosine residues that then serve as binding sites for scaffold proteins and effectors. Ligand specificity and signaling is modulated by alternative splicing, proteolytic processing, and by the formation of heterodimers with other ERBB family members, thereby creating multiple combinations of intracellular phosphotyrosines that trigger ligand- and context-specific cellular responses. Mediates phosphorylation of SHC1 and activation of the MAP kinases MAPK1/ERK2 and MAPK3/ERK1. Isoform JM-A CYT-1 and isoform JM-B CYT-1 phosphorylate PIK3R1, leading to the activation of phosphatidylinositol 3-kinase and AKT1 and protect cells against apoptosis. Isoform JM-A CYT-1 and isoform JM-B CYT-1 mediate reorganization of the actin cytoskeleton and promote cell migration in response to NRG1. Isoform JM-A CYT-2 and isoform JM-B CYT-2 lack the phosphotyrosine that mediates interaction with PIK3R1, and hence do not phosphorylate PIK3R1, do not protect cells against apoptosis, and do not promote reorganization of the actin cytoskeleton and cell migration. Proteolytic processing of isoform JM-A CYT-1 and isoform JM-A CYT-2 gives rise to the corresponding soluble intracellular domains (4ICD) that translocate to the nucleus, promote nuclear import of STAT5A, activation of STAT5A, mammary epithelium differentiation, cell proliferation and activation of gene expression. The ERBB4 soluble intracellular domains (4ICD) colocalize with STAT5A at the CSN2 promoter to regulate transcription of milk proteins during lactation. The ERBB4 soluble intracellular domains can also translocate to mitochondria and promote apoptosis.

NCBI and Uniprot Product Information

• NCBI GI #: 3913590
• NCBI GeneID: 2066
• UniProt Accession #: Q15303; Q2M1W1; Q59EW4; B7ZLD7; B7ZLE2; B7ZLE3
• Molecular Weight: 143,968 Da
• NCBI Official Full Name: Receptor tyrosine-protein kinase erbB-4
• NCBI Official Synonym Full Names: erb-b2 receptor tyrosine kinase 4
• NCBI Official Symbol: ERBB4
• NCBI Official Synonym Symbols: HER4; ALS19; p180erbB4
• NCBI Protein Information: receptor tyrosine-protein kinase erbB-4
• UniProt Protein Name: Receptor tyrosine-protein kinase erbB-4
• UniProt Gene Name: ERBB4
• UniProt Synonym Gene Names: HER4; 4ICD; E4ICD
• UniProt Entry Name: ERBB4_HUMAN

NCBI Description

This gene is a member of the Tyr protein kinase family and the epidermal growth factor receptor subfamily. It encodes a single-pass type I membrane protein with multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. The protein binds to and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. Multiple proteolytic events allow for the release of a cytoplasmic fragment and an extracellular fragment. Mutations in this gene have been associated with cancer. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

HER4: a receptor tyrosine kinase of the EGFR family. Specifically binds and is activated by neuregulins, NRG- 2, NRG-3, heparin-binding EGF-like growth factor, betacellulin and NTAK. Heterodimerizes and signals with other EGF receptors. Interaction with these factors induces cell differentiation. Not activated by EGF, TGF-A, and amphiregulin. Interacts with PDZ domains of DLG2, DLG3, DLG4 and the syntrophin SNTB2. Interacts with WWOX. May act as a tumor suppressor: overexpressed in head and neck cancer , but downregulated in renal cancer, papillary carcinoma, high-grade gliomas and invasive breast cancer. 3 alternatively-spliced isoforms of the human protein have been reported.

Protein type: Protein kinase, TK; EC 2.7.10.1; Membrane protein, integral; Protein kinase, tyrosine (receptor); Kinase, protein; TK group; EGFR family

Chromosomal Location of Human Ortholog: 2q33.3-q34

Cellular Component: basolateral plasma membrane; cytosol; extracellular region; integral to membrane; mitochondrial matrix; mitochondrion; nucleoplasm; nucleus; plasma membrane; receptor complex

Molecular Function: ATP binding; epidermal growth factor receptor binding; phosphatidylinositol-4,5-bisphosphate 3-kinase activity; protein binding; protein homodimerization activity; protein-tyrosine kinase activity; Ras guanyl-nucleotide exchange factor activity; receptor signaling protein tyrosine kinase activity; transmembrane receptor protein tyrosine kinase activity

Biological Process: cell fate commitment; cell migration; cell proliferation; central nervous system morphogenesis; embryonic pattern specification; heart development; lactation; MAPKKK cascade; mitochondrial fragmentation during apoptosis; negative regulation of apoptosis; negative regulation of cell proliferation; nervous system development; neural crest cell migration; olfactory bulb interneuron differentiation; peptidyl-tyrosine phosphorylation; phosphoinositide phosphorylation; phosphoinositide-mediated signaling; positive regulation of cardiac muscle cell proliferation; positive regulation of cell proliferation; positive regulation of GTPase activity; positive regulation of phosphoinositide 3-kinase activity; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein amino acid phosphorylation; positive regulation of transcription, DNA-dependent; positive regulation of tyrosine phosphorylation of Stat5 protein; protein amino acid autophosphorylation; regulation of cell migration; regulation of phosphoinositide 3-kinase cascade; signal transduction; transcription, DNA-dependent; transmembrane receptor protein tyrosine kinase signaling pathway

Disease: Amyotrophic Lateral Sclerosis 19