Deoxyribonuclease II, Porcine spleen Enzyme | DNASE2 enzyme

Deoxyribonuclease II, Porcine spleen (DNase II)

Cat. Num. AAA653670

• Purity: Molecular Biology Grade; Chromatographically purified in a modification of the procedure of Bernardi, et al., BBA, 129, 1 (1966).
• Synonyms: Deoxyribonuclease II; Porcine spleen; Porcine spleen (DNase II); DNASE2 enzyme

• Host: Porcine pancreas
• Purity/Purification: Molecular Biology Grade; Chromatographically purified in a modification of the procedure of Bernardi, et al., BBA, 129, 1 (1966).
• Form/Format: Supplied as a chromatographically purified, dialyzed, lyophilized powder.

• Application Notes: DNA Sequencing (acidic pH), DNA Digestion (acidic pH)
• CAS Number: 9003-98-9
• Activity: U/mg: 39,912; dw: u/mgP: 46,409
• Country of Origin: US certified starting material, collected in USDA approved facilities
• % Protein: 86%
• Unit Definition: 1 unit causes an increase in absorbance at 260nm of 0.001 per minute per minute at 25 degree C, pH4.6 using highly polymerized DNA as substate.
• Quality Control: SDS-PAGE
• Unit Definition: 1 unit causes an increase in absorbance at 260 nm of 0.002 per minute per ml at 25ºC when acting upon highly polymerized DNA at pH 4.6.; Note: 0.005 Kunitz units digests 1ug DNA in 10 minutes at 37 degree C, 50mM Tris, 1mM Ca+2, 1mM Mg+2 in a 50ul reaction.
• Preparation and Storage: Lyophilized powder may be stored at -20 degree C. Stable for 6 months after receipt at -20 degree C. For maximum recofery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for DNASE2 enzyme

Deoxyribonuclease II is a glycoprotein endonuclease with a dimeric structure. Deoxyribonuclease II from porcine spleen has a molecular weight of 38kD. Optimum pH range is 4.5-5.0 at ionic strength 0.15M. Deoxyribonuclease II (Acid DNase) hydrolyzes deoxyribonucleotide linkages in native and denatured DNA yielding products with 3'-phosphates. It also acts on p-nitrophenylphosphodiesters at pH 5.6-5.9. Bernardi (BBRC, 17, 573, 1971) describes a three stage degradation of native DNA by DNase II.

Product Categories/Family for DNASE2 enzyme

Cloning; Cloning-Enzymes

NCBI and Uniprot Product Information

• NCBI GI #: 3157444
• NCBI GeneID: 397398
• UniProt Accession #: O62855
• Molecular Weight: 38kD
• NCBI Official Full Name: Deoxyribonuclease II
• NCBI Official Symbol: DNASE2
• NCBI Protein Information: deoxyribonuclease-2-alpha; acid DNase; DNase II alpha; lysosomal DNase II; deoxyribonuclease II alpha
• UniProt Protein Name: Deoxyribonuclease-2-alpha
• Protein Family: Deoxyribonuclease
• UniProt Gene Name: DNASE2
• UniProt Synonym Gene Names: DNASE2A; DNL2; DNase II alpha
• UniProt Entry Name: DNS2A_PIG

Uniprot Description

Function: Hydrolyzes DNA under acidic conditions with a preference for double-stranded DNA. Plays a major role in the degradation of nuclear DNA in cellular apoptosis during development. Necessary for proper fetal development and for definitive erythropoiesis in fetal liver, where it degrades nuclear DNA expelled from erythroid precursor cells

By similarity.

Catalytic activity: Endonucleolytic cleavage to nucleoside 3'-phosphates and 3'-phosphooligonucleotide end-products.

Subcellular location: Lysosome.

Miscellaneous: Not required for the generation of the characteristic DNA fragmentation observed in apoptotic cells, but for the degradation of DNA from dying cells.

Sequence similarities: Belongs to the DNase II family.