Porcine TBG ELISA Kit | TBG elisa kit

Porcine TBG (Thyroxine Binding Globulin) ELISA Kit

Cat. Num. AAA2511842

• Gene Names: SERPINA7; TBG
• Reactivity: Porcine
• Synonyms: TBG; Porcine TBG (Thyroxine Binding Globulin) ELISA Kit; TBG elisa kit

• Reactivity: Porcine
• Specificity: This kit recognizes natural and recombinant Porcine TBG. No significant cross-reactivity or interference between Porcine TBG and analogues was observed.
• Sequence Length: 415

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 0.781-50ng/mL
• Sensitivity: Min: 0.469ng/mL; Max: 50ng/mL
• Preparation and Storage: Store at 4 degree C.

Related Product Information for TBG elisa kit

Intended Uses: This ELISA kit applies to the in vitro quantitative determination of Porcine TBG concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to TBG. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for TBG and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain TBG, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of TBG. You can calculate the concentration of TBG in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 205277441
• NCBI GeneID: 6906
• NCBI Accession #: NP_000345.2
• NCBI GenBank Nucleotide #: NM_000354.5
• UniProt Accession #: P05543; D3DUX1
• Molecular Weight: 46,325 Da
• NCBI Official Full Name: thyroxine-binding globulin
• NCBI Official Synonym Full Names: serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 7
• NCBI Official Symbol: SERPINA7
• NCBI Official Synonym Symbols: TBG
• NCBI Protein Information: thyroxine-binding globulin; T4-binding globulin; serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 7; serpin A7; thyroxin-binding globulin
• UniProt Protein Name: Thyroxine-binding globulin
• UniProt Gene Name: SERPINA7
• UniProt Synonym Gene Names: TBG
• UniProt Entry Name: THBG_HUMAN

NCBI Description

There are three proteins including thyroxine-binding globulin (TBG), transthyretin and albumin responsible for carrying the thyroid hormones thyroxine (T4) and 3,5,3'-triiodothyronine (T3) in the bloodstream. This gene encodes the major thyroid hormone transport protein, TBG, in serum. It belongs to the serpin family in genomics, but the protein has no inhibitory function like many other members of the serpin family. Mutations in this gene result in TGB deficiency, which has been classified as partial deficiency, complete deficiency, and excess, based on the level of serum TBG. Alternatively spliced transcript variants encoding different isoforms have been found, but the full-length nature of these variants has not been determined.[provided by RefSeq, Jun 2012]

Uniprot Description

SERPINA7: Major thyroid hormone transport protein in serum. Defects in SERPINA7 are a cause of thyroxine-binding globulin deficiency (TBG deficiency). Mutations in the SERPINA7 gene can result as a whole spectrum of deficiencies, characterized by either reduced or increased TBG levels in the serum. Patients show, respectively, reduced or elevated protein- bound iodine but are euthyroid. Belongs to the serpin family.

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: Xq22.2

Cellular Component: extracellular space; extracellular region

Molecular Function: serine-type endopeptidase inhibitor activity; hormone binding

Biological Process: response to drug; response to peptide hormone stimulus; aging; response to vitamin A; post-embryonic development; response to corticosterone stimulus