Porcine Syndecan 1 (SDC1) ELISA Kit | SDC1 elisa kit

Pig Syndecan 1 (SDC1) ELISA Kit

Cat. Num. AAA2703970

• Gene Names: SDC1; SDC; CD138; SYND1; syndecan
• Reactivity: Porcine
• Synonyms: Syndecan 1 (SDC1); Pig Syndecan 1 (SDC1) ELISA Kit; CD138; SDC; SYND1; Syndecan Proteoglycan 1; SDC1 elisa kit

• Reactivity: Porcine
• Specificity: This assay has high sensitivity and excellent specificity for detection of Syndecan 1 (SDC1).; No significant cross-reactivity or interference between Syndecan 1 (SDC1) and analogues was observed.
• Sequence Length: 310

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.057ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Syndecan 1 (SDC1) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Syndecan 1 (SDC1) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for SDC1 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Syndecan 1 (SDC1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Syndecan 1 (SDC1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Syndecan 1 (SDC1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Syndecan 1 (SDC1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 55749480
• NCBI GeneID: 6382
• NCBI Accession #: NP_001006947.1
• NCBI GenBank Nucleotide #: NM_001006946.1
• UniProt Accession #: P18827; Q53QV0; Q546D3; Q96HB7; D6W523
• Molecular Weight: 32,462 Da
• NCBI Official Full Name: syndecan-1
• NCBI Official Synonym Full Names: syndecan 1
• NCBI Official Symbol: SDC1
• NCBI Official Synonym Symbols: SDC; CD138; SYND1; syndecan
• NCBI Protein Information: syndecan-1
• UniProt Protein Name: Syndecan-1
• Protein Family: Syndecan
• UniProt Gene Name: SDC1
• UniProt Synonym Gene Names: SDC; SYND1

NCBI Description

The protein encoded by this gene is a transmembrane (type I) heparan sulfate proteoglycan and is a member of the syndecan proteoglycan family. The syndecans mediate cell binding, cell signaling, and cytoskeletal organization and syndecan receptors are required for internalization of the HIV-1 tat protein. The syndecan-1 protein functions as an integral membrane protein and participates in cell proliferation, cell migration and cell-matrix interactions via its receptor for extracellular matrix proteins. Altered syndecan-1 expression has been detected in several different tumor types. While several transcript variants may exist for this gene, the full-length natures of only two have been described to date. These two represent the major variants of this gene and encode the same protein. [provided by RefSeq, Jul 2008]

Uniprot Description

syndecan-1: Cell surface proteoglycan that bears both heparan sulfate and chondroitin sulfate and that links the cytoskeleton to the interstitial matrix. Belongs to the syndecan proteoglycan family.

Protein type: Extracellular matrix; Membrane protein, integral; Motility/polarity/chemotaxis

Chromosomal Location of Human Ortholog: 2p24.1

Cellular Component: cell surface; external side of plasma membrane; extracellular exosome; Golgi lumen; integral component of plasma membrane; lysosomal lumen; plasma membrane; protein complex

Molecular Function: identical protein binding; protein binding; protein C-terminus binding

Biological Process: cell migration; glycosaminoglycan biosynthetic process; glycosaminoglycan catabolic process; glycosaminoglycan metabolic process; inflammatory response; leukocyte migration; myoblast development; odontogenesis; response to calcium ion; response to cAMP; response to glucocorticoid stimulus; response to hydrogen peroxide; response to toxin; retinoid metabolic process; Sertoli cell development; striated muscle cell development; ureteric bud development; Wnt receptor signaling pathway through beta-catenin; wound healing