Porcine Protein N-terminal asparagine amidohydrolase (NTAN1) ELISA Kit | NTAN1 elisa kit

Porcine Protein N-terminal asparagine amidohydrolase (NTAN1) ELISA Kit

Cat. Num. AAA7218404

• Gene Names: NTAN1; PNAA; PNAD
• Reactivity: Porcine
• Synonyms: Protein N-terminal asparagine amidohydrolase (NTAN1); Porcine Protein N-terminal asparagine amidohydrolase (NTAN1) ELISA Kit; NTAN1 elisa kit

• Reactivity: Porcine

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 ng/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve

Related Product Information for NTAN1 elisa kit

Intended Uses: This NTAN1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine NTAN1. This ELISA kit for research use only!

Principle of the Assay||NTAN1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-NTAN1 antibody and an NTAN1-HRP conjugate. The assay sample and buffer are incubated together with NTAN1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the NTAN1 concentration since NTAN1 from samples and NTAN1-HRP conjugate compete for the anti-NTAN1 antibody binding site. Since the number of sites is limited, as more sites are occupied by NTAN1 from the sample, fewer sites are left to bind NTAN1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The NTAN1 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for NTAN1 elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 399124762
• NCBI GeneID: 123803
• NCBI Accession #: NP_001257696.1
• NCBI GenBank Nucleotide #: NM_001270767.1
• Molecular Weight: 34,677 Da
• NCBI Official Full Name: protein N-terminal asparagine amidohydrolase isoform 2
• NCBI Official Synonym Full Names: N-terminal asparagine amidase
• NCBI Official Symbol: NTAN1
• NCBI Official Synonym Symbols: PNAA; PNAD
• NCBI Protein Information: protein N-terminal asparagine amidohydrolase; protein NTN-amidase; protein N-terminal Asn amidase; protein N-terminal asparagine amidase; protein NH2-terminal asparagine deamidase
• UniProt Protein Name: Protein N-terminal asparagine amidohydrolase
• Protein Family: Protein N-terminal asparagine amidohydrolase
• UniProt Gene Name: NTAN1
• UniProt Synonym Gene Names: PNAA; PNAD; Protein N-terminal Asn amidase; Protein N-terminal asparagine amidase; Protein NTN-amidase
• UniProt Entry Name: NTAN1_HUMAN

NCBI Description

The protein encoded by this gene functions in a step-wise process of protein degradation through the N-end rule pathway. This protein acts as a tertiary destabilizing enzyme that deamidates N-terminal L-Asn residues on proteins to produce N-terminal L-Asp. L-Asp substrates are subsequently conjugated to L-Arg, which is recognized by specific E3 ubiquitin ligases and targeted to the proteasome. Pseudogenes of this gene are located on the long arms of chromosomes 8, 10 and 12. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jul 2012]

Uniprot Description

NTAN1: Side-chain deamidation of N-terminal asparagine residues to aspartate. Required for the ubiquitin-dependent turnover of intracellular proteins that initiate with Met-Asn. These proteins are acetylated on the retained initiator methionine and can subsequently be modified by the removal of N-acetyl methionine by acylaminoacid hydrolase (AAH). Conversion of the resulting N- terminal asparagine to aspartate by PNAD renders the protein susceptible to arginylation, polyubiquitination and degradation as specified by the N-end rule. This enzyme does not act on substrates with internal or C-terminal asparagines and does not act on glutamine residues in any position.

Protein type: EC 3.5.1.-; Hydrolase

Chromosomal Location of Human Ortholog: 16p13.11

Cellular Component: cytoplasm; nucleus

Molecular Function: protein N-terminal asparagine amidohydrolase activity

Biological Process: metabolic process; adult locomotory behavior; memory