Porcine NRP1 ELISA Kit | NRP1 elisa kit
Porcine NRP1 (Neuropilin 1) ELISA Kit
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to NRP1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for NRP1and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain NRP1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of NRP1.You can calculate the concentration of NRP1in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
This gene encodes one of two neuropilins, which contain specific protein domains which allow them to participate in several different types of signaling pathways that control cell migration. Neuropilins contain a large N-terminal extracellular domain, made up of complement-binding, coagulation factor V/VIII, and meprin domains. These proteins also contains a short membrane-spanning domain and a small cytoplasmic domain. Neuropilins bind many ligands and various types of co-receptors; they affect cell survival, migration, and attraction. Some of the ligands and co-receptors bound by neuropilins are vascular endothelial growth factor (VEGF) and semaphorin family members. Several alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. [provided by RefSeq, Oct 2011]
Uniprot Description
NRP1: The membrane-bound isoform 1 is a receptor involved in the development of the cardiovascular system, in angiogenesis, in the formation of certain neuronal circuits and in organogenesis outside the nervous system. It mediates the chemorepulsant activity of semaphorins. It binds to semaphorin 3A, The PLGF-2 isoform of PGF, The VEGF-165 isoform of VEGF and VEGF-B. Coexpression with KDR results in increased VEGF-165 binding to KDR as well as increased chemotaxis. It may regulate VEGF-induced angiogenesis. Homodimer, and heterodimer with NRP2. Interacts with FER. Binds PLXNB1. The expression of isoforms 1 and 2 does not seem to overlap. Isoform 1 is expressed by the blood vessels of different tissues. In the developing embryo it is found predominantly in the nervous system. In adult tissues, it is highly expressed in heart and placenta; moderately in lung, liver, skeletal muscle, kidney and pancreas; and low in adult brain. Isoform 2 is found in liver hepatocytes, kidney distal and proximal tubules. Belongs to the neuropilin family. 2 isoforms of the human protein are produced by alternative splicing.
Protein type: Receptor, misc.; Membrane protein, integral
Chromosomal Location of Human Ortholog: 10p12
Cellular Component: extracellular space; growth cone; focal adhesion; cell surface; cell soma; axon; early endosome; integral to membrane; neurofilament; plasma membrane; cytoplasmic vesicle; cytosol; receptor complex
Molecular Function: heparin binding; vascular endothelial growth factor receptor activity; protein binding; semaphorin receptor activity; growth factor binding; cytokine binding; metal ion binding; coreceptor activity
Biological Process: axon guidance; facial nerve structural organization; neuron migration; vestibulocochlear nerve structural organization; platelet-derived growth factor receptor signaling pathway; signal transduction; negative regulation of axon extension involved in axon guidance; positive regulation of smooth muscle cell migration; gonadotrophin-releasing hormone neuronal migration to the hypothalamus; positive chemotaxis; cell-cell signaling; branchiomotor neuron axon guidance; dendrite development; response to wounding; angiogenesis; negative regulation of neuron apoptosis; trigeminal nerve structural organization; axonal fasciculation; hepatocyte growth factor receptor signaling pathway; axon extension involved in axon guidance; patterning of blood vessels; organ morphogenesis; positive regulation of peptidyl-tyrosine phosphorylation; cell migration during sprouting angiogenesis; otic placode formation; positive regulation of axon extension involved in axon guidance; artery morphogenesis; positive regulation of endothelial cell proliferation; nerve development; sprouting angiogenesis; vascular endothelial growth factor receptor signaling pathway; retinal ganglion cell axon guidance
Research Articles on NRP1
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Product Notes
The Porcine NRP1 nrp1 (Catalog #AAA2509640) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2509640 ELISA Kit recognizes Porcine NRP1. It is sometimes possible for the material contained within the vial of "NRP1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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