Typical Testing Data/Standard Curve (for reference only)
Porcine Myogenic factor 6 (MYF6) ELISA Kit | MYF6 elisa kit
Porcine Myogenic factor 6 (MYF6) ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: MXD1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-MXD1 antibody and an MXD1-HRP conjugate. The assay sample and buffer are incubated together with MXD1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the MXD1 concentration since MXD1 from samples and MXD1-HRP conjugate compete for the anti-MXD1 antibody binding site. Since the number of sites is limited, as more sites are occupied by MXD1 from the sample, fewer sites are left to bind MXD1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The MXD1 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
The protein encoded by this gene is a probable basic helix-loop-helix (bHLH) DNA binding protein involved in muscle differentiation. The encoded protein likely acts as a heterodimer with another bHLH protein. Defects in this gene are a cause of autosomal dominant centronuclear myopathy (ADCNM). [provided by RefSeq, May 2010]
Uniprot Description
Myf-6: Involved in muscle differentiation (myogenic factor). Induces fibroblasts to differentiate into myoblasts. Probable sequence specific DNA-binding protein. Defects in MYF6 may be a cause of centronuclear myopathy type 3 (CNM3). A congenital muscle disorder characterized by progressive muscular weakness and wasting involving mainly limb girdle, trunk, and neck muscles. It may also affect distal muscles. Weakness may be present during childhood or adolescence or may not become evident until the third decade of life. Ptosis is a frequent clinical feature. The most prominent histopathologic features include high frequency of centrally located nuclei in muscle fibers not secondary to regeneration, radial arrangement of sarcoplasmic strands around the central nuclei, and predominance and hypotrophy of type 1 fibers.
Protein type: Transcription factor; DNA-binding
Chromosomal Location of Human Ortholog: 12q21
Cellular Component: nucleoplasm; nucleus
Molecular Function: RNA polymerase II transcription factor activity, enhancer binding; protein heterodimerization activity; transcription factor activity
Biological Process: regulation of transcription from RNA polymerase II promoter; transcription from RNA polymerase II promoter; somitogenesis; skeletal muscle development; muscle cell differentiation; positive regulation of skeletal muscle fiber development; muscle cell fate commitment; skeletal muscle regeneration; positive regulation of muscle cell differentiation; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription, DNA-dependent; positive regulation of myoblast differentiation
Disease: Myopathy, Centronuclear, 3
Research Articles on MYF6
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Product Notes
The Porcine MYF6 myf6 (Catalog #AAA7214838) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7214838 ELISA Kit recognizes Porcine MYF6. It is sometimes possible for the material contained within the vial of "Myogenic factor 6 (MYF6), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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