Porcine IL-4 ELISA Kit | IL-4 elisa kit

Porcine IL-4 (Interleukin 4) ELISA Kit

Cat. Num. AAA2513320

• Reactivity: Porcine
• Synonyms: IL-4; Porcine IL-4 (Interleukin 4) ELISA Kit; IL-4 elisa kit

• Reactivity: Porcine
• Specificity: This assay has high sensitivity and excellent specificity for detection of Porcine IL-4. No significant cross-reactivity or interference between Porcine IL-4 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Porcine IL-4 and all the analogues, therefore, cross reaction may still exist.
• Sequence Length: 133

• Samples: Serum, Plasma, Biological Fluids
• Assay Type: Sandwich
• Detection Range: 78.125-5000pg/mL
• Sensitivity: Min: 46.875pg/mL; Max: 5000pg/mL
• Preparation and Storage: Store at 4 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL-4 elisa kit

Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of IL-4 in Porcine serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Porcine IL-4. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Porcine IL-4 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Porcine IL-4, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Porcine IL-4. You can calculate the concentration of Porcine IL-4 in the samples by comparing the OD of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 55742622
• NCBI GeneID: 397225
• NCBI Accession #: NP_999288.1
• NCBI GenBank Nucleotide #: NM_214123.1
• UniProt Accession #: Q04745; Q29054; Q8MKE6
• Molecular Weight: 15,023 Da
• NCBI Official Full Name: interleukin-4
• NCBI Official Symbol: IL4
• NCBI Protein Information: interleukin-4; B-cell stimulatory factor 1; BSF-1; IL-4; lymphocyte stimulatory factor 1
• UniProt Protein Name: Interleukin-4
• UniProt Gene Name: IL4
• UniProt Synonym Gene Names: IL-4; BSF-1
• UniProt Entry Name: IL4_PIG

Uniprot Description

Participates in at least several B-cell activation processes as well as of other cell types. It is a costimulator of DNA-synthesis. It induces the expression of class II MHC molecules on resting B-cells. It enhances both secretion and cell surface expression of IgE and IgG1. It also regulates the expression of the low affinity Fc receptor for IgE (CD23) on both lymphocytes and monocytes. Positively regulates IL31RA expression in macrophages.