Porcine GDI1 ELISA Kit | GDI1 elisa kit
Porcine GDI1 (GDP Dissociation Inhibitor 1) ELISA Kit
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to GDI1. Standards or samples are added to the appropriate micro ELISA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for GDI1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain GDI1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of GDI1. You can calculate the concentration of GDI1 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI Description
GDP dissociation inhibitors are proteins that regulate the GDP-GTP exchange reaction of members of the rab family, small GTP-binding proteins of the ras superfamily, that are involved in vesicular trafficking of molecules between cellular organelles. GDIs slow the rate of dissociation of GDP from rab proteins and release GDP from membrane-bound rabs. GDI1 is expressed primarily in neural and sensory tissues. Mutations in GDI1 have been linked to X-linked nonspecific mental retardation. [provided by RefSeq, Jul 2008]
Uniprot Description
GDI1: a GDP dissociation inhibitor protein expressed primarily in neural and sensory tissues. GDP dissociation inhibitors are proteins that regulate the GDP-GTP exchange reaction of members of the Rab family, small GTP-binding proteins of the Ras superfamily, that are involved in vesicular trafficking of molecules between cellular organelles. GDIs slow the rate of dissociation of GDP from Rab proteins and release GDP from membrane-bound Rabs. Mutations have been linked to X-linked nonspecific mental retardation.
Protein type: G protein regulator, misc.; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: Xq28
Cellular Component: Golgi apparatus; neuron projection; protein complex; cytoplasm; midbody; cytosol
Molecular Function: protein binding; GDP-dissociation inhibitor activity; Rab GDP-dissociation inhibitor activity; GTPase activator activity; Rab GTPase binding
Biological Process: protein transport; regulation of small GTPase mediated signal transduction; small GTPase mediated signal transduction; negative regulation of axonogenesis; response to calcium ion; Rab protein signal transduction; signal transduction; positive regulation of GTPase activity
Disease: Mental Retardation, X-linked 41
Research Articles on GDI1
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Product Notes
The Porcine GDI1 gdi1 (Catalog #AAA2509770) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2509770 ELISA Kit recognizes Porcine GDI1. It is sometimes possible for the material contained within the vial of "GDI1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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