Porcine Eotaxin ELISA Kit | CCL11 elisa kit

Porcine Eotaxin ELISA Kit

Cat. Num. AAA263441

• Gene Names: CCL11; SCYA11
• Reactivity: Porcine
• Synonyms: Eotaxin; Porcine Eotaxin ELISA Kit; CCL11 elisa kit

• Reactivity: Porcine
• Specificity: No cross-reaction with other factors.
• Sequence Length: 97

• Samples: Porcine serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 1000 pg/ml-15.6 pg/ml
• Sensitivity: Up to 5 pg/ml
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CCL11 elisa kit

Principle of the Assay: This experiment use double-sandwich elisa technique and the ELISA Kit provided is typical. The pre-coated antibody is Porcine Eotaxin monoclonal antibody and the detecting antibody is polyclonal antibody with biotin labeled. Samples and biotin labeling antibody are added into ELISA plate wells and washed out with PBS or TBS. Then Avidin-peroxidase conjugates are added to ELISA wells in order; Use TMB substrate for coloring after reactant thoroughly washed out by PBS or TBS. TMB turns into blue in peroxidase catalytic and finally turns into yellow under the action of acid. The color depth and the testing factors in samples are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 4506827
• NCBI GeneID: 6356
• NCBI Accession #: NP_002977.1
• NCBI GenBank Nucleotide #: NM_002986.2
• Molecular Weight: 10,732 Da
• NCBI Official Full Name: eotaxin
• NCBI Official Synonym Full Names: chemokine (C-C motif) ligand 11
• NCBI Official Symbol: CCL11
• NCBI Official Synonym Symbols: SCYA11
• NCBI Protein Information: eotaxin; eotaxin-1; eosinophil chemotactic protein; small inducible cytokine subfamily A (Cys-Cys), member 11 (eotaxin)
• UniProt Protein Name: Eotaxin
• Protein Family: Eotaxin
• UniProt Gene Name: CCL11
• UniProt Synonym Gene Names: SCYA11
• UniProt Entry Name: CCL11_HUMAN

NCBI Description

This antimicrobial gene is one of several chemokine genes clustered on the q-arm of chromosome 17. Chemokines form a superfamily of secreted proteins involved in immunoregulatory and inflammatory processes. The superfamily is divided into four subfamilies based on the arrangement of the N-terminal cysteine residues of the mature peptide. This chemokine, a member of the CC subfamily, displays chemotactic activity for eosinophils, but not mononuclear cells or neutrophils. This eosinophil-specific chemokine is thought to be involved in eosinophilic inflammatory diseases such as atopic dermatitis, allergic rhinitis, asthma and parasitic infections. [provided by RefSeq, Sep 2014]

Uniprot Description

CCL11: In response to the presence of allergens, this protein directly promotes the accumulation of eosinophils, a prominent feature of allergic inflammatory reactions. Binds to CCR3. Belongs to the intercrine beta (chemokine CC) family.

Protein type: Motility/polarity/chemotaxis; Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 17q12

Cellular Component: extracellular space; extracellular region

Molecular Function: protein binding; chemokine activity

Biological Process: chronic inflammatory response; mast cell chemotaxis; response to virus; actin filament organization; cytoskeleton organization and biogenesis; signal transduction; chemotaxis; protein amino acid phosphorylation; cellular calcium ion homeostasis; regulation of cell shape; positive regulation of angiogenesis; response to radiation; positive regulation of actin filament polymerization; eosinophil chemotaxis; immune response; positive regulation of endothelial cell proliferation; inflammatory response; cell adhesion; positive regulation of cell migration

Disease: Asthma, Susceptibility To; Human Immunodeficiency Virus Type 1, Susceptibility To