Porcine DMT1 (Divalent metal transporter 1) ELISA Kit | DMT1 elisa kit

Porcine DMT1 (Divalent metal transporter 1) ELISA Kit

Cat. Num. AAA7606135

• Gene Names: slc11a2; DMT1; cb426; fa07b10; wu:fa07b10; zgc:136699
• Reactivity: Porcine
• Synonyms: DMT1 (Divalent metal transporter 1); Porcine DMT1 (Divalent metal transporter 1) ELISA Kit; SLC11A2; DCT1; DMT-1; NRAMP2; Solute carrier family 11 member 2; Divalent cation transporter 1; Natural resistance-associated macrophage protein 2; DMT1 elisa kit

• Reactivity: Porcine
• Specificity: This assay has high sensitivity and excellent specificity for detection of DMT1 . No significant cross-reactivity or interference between DMT1 and analogues was observed.
• Sequence Length: 547

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: <0.094ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level DMT1 were tested 20 times on one plate, respectively. Intra-Assay: CV<8%
• Inter-assay Precision: Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level DMT1 were tested on 3 different plates, 8 replicates in each plate. CV (%) = SD/meanX100. Inter-Assay: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DMT1 elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- DMT1 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- DMT1 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRPStreptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the DMT1 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of DMT1 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 27475929
• NCBI GeneID: 678623
• Molecular Weight: 60,107 Da
• NCBI Official Full Name: divalent metal transporter 1
• NCBI Official Synonym Full Names: solute carrier family 11 (proton-coupled divalent metal ion transporter), member 2
• NCBI Official Symbol: slc11a2
• NCBI Official Synonym Symbols: DMT1; cb426; fa07b10; wu:fa07b10; zgc:136699
• NCBI Protein Information: natural resistance-associated macrophage protein 2
• UniProt Protein Name: Divalent metal transporter 1
• UniProt Gene Name: slc11a2