Porcine Dehydroepiandrosterone (DHEA) ELISA Kit | DHEA elisa kit

Porcine Dehydroepiandrosterone (DHEA) ELISA Kit

Cat. Num. AAA264006

• Gene Names: SULT2A1; HST; ST2; STD; hSTa; DHEAS; ST2A1; ST2A3; DHEA-ST
• Reactivity: Porcine
• Synonyms: Dehydroepiandrosterone (DHEA); Porcine Dehydroepiandrosterone (DHEA) ELISA Kit; DHEA elisa kit

• Reactivity: Porcine
• Sequence Length: 285

• Samples: Porcine serum, plasma or cell culture supernatant
• Assay Type: Quantitative Sandwich
• Detection Range: 10 ng/mL-0.156 ng/mL
• Sensitivity: Up to 0.05 ng/mL
• Intra-assay Precision: <= 8%
• Inter-assay Precision: <= 12%
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for DHEA elisa kit

Principle of the Assay: As mentioned above, this kit utilizes the Double Antibody Sandwich ELISA technique. The pre-coated antibody is an anti-Porcine DHEA monoclonal antibody, while the detection antibody is a biotinylated polyclonal antibody. Samples and biotinylated antibodies are added into ELISA plate wells and washed out with PBS or TBS after their respective additions to the wells. Then Avidin-peroxidase conjugates are added to the wells in after. TMB substrate is used for coloration after the enzyme conjugate has already been thoroughly washed out of the wells by PBS or TBS. TMB reacts to form a blue product from the peroxidase activity, and finally turns to yellow after addition of the stop solution (Color Reagent C). The color intensity and quantity of target analyte in the sample are positively correlated.

NCBI and Uniprot Product Information

• NCBI GI #: 468253
• NCBI GeneID: 6822
• Molecular Weight: 33,780 Da
• NCBI Official Full Name: dehydroepiandrosterone sulfotransferase
• NCBI Official Synonym Full Names: sulfotransferase family, cytosolic, 2A, dehydroepiandrosterone (DHEA)-preferring, member 1
• NCBI Official Symbol: SULT2A1
• NCBI Official Synonym Symbols: HST; ST2; STD; hSTa; DHEAS; ST2A1; ST2A3; DHEA-ST
• NCBI Protein Information: bile salt sulfotransferase; bile-salt sulfotranasferase 2A1; alcohol/hydroxysteroid sulfotransferase
• UniProt Protein Name: Bile salt sulfotransferase
• UniProt Gene Name: SULT2A1
• UniProt Synonym Gene Names: HST; STD; DHEA-ST; HST; ST2A1
• UniProt Entry Name: ST2A1_HUMAN

NCBI Description

This gene encodes a member of the sulfotransferase family. Sulfotransferases aid in the metabolism of drugs and endogenous compounds by converting these substances into more hydrophilic water-soluble sulfate conjugates that can be easily excreted. This protein catalyzes the sulfation of steroids and bile acids in the liver and adrenal glands, and may have a role in the inherited adrenal androgen excess in women with polycystic ovary syndrome. [provided by RefSeq, Mar 2010]

Uniprot Description

SULT2A1: Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfonation of steroids and bile acids in the liver and adrenal glands. Belongs to the sulfotransferase 1 family.

Protein type: EC 2.8.2.14; Transferase

Chromosomal Location of Human Ortholog: 19q13.3

Cellular Component: cytosol

Molecular Function: sulfotransferase activity; protein binding; bile-salt sulfotransferase activity

Biological Process: steroid metabolic process; xenobiotic metabolic process; bile acid catabolic process; digestion; sulfation; cellular lipid metabolic process; 3'-phosphoadenosine 5'-phosphosulfate metabolic process