Porcine COL10 ELISA Kit | COL10 elisa kit
Porcine COL10 (Collagen Type X) ELISA Kit
Gene Names
Col10a1; Col10; Col10a-1
Reactivity
Porcine
Synonyms
COL10; Porcine COL10 (Collagen Type X) ELISA Kit; COL10 elisa kit
Reactivity
Porcine
Specificity
This assay has high sensitivity and excellent specificity for detection of Porcine COL10. No significant cross-reactivity or interference between Porcine COL10 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Porcine COL10 and all the analogues, therefore, cross reaction may still exist.
Sequence Length
680
Samples
Serum, Plasma, Biological Fluids
Assay Type
Sandwich
Detection Range
0.313-20ng/mL
Sensitivity
Min: 0.188ng/mL; Max: 20ng/mL
Preparation and Storage
Store at 4 degree C.
Related Product Information for COL10 elisa kit
Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of COL10 in Porcine serum, plasma and other biological fluids
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Porcine COL10. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Porcine COL10 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Porcine COL10, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Porcine COL10. You can calculate the concentration of Porcine COL10 in the samples by comparing the OD of the samples to the standard curve.
Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Porcine COL10. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Porcine COL10 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Porcine COL10, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Porcine COL10. You can calculate the concentration of Porcine COL10 in the samples by comparing the OD of the samples to the standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
66,775 Da
NCBI Official Full Name
collagen alpha-1(X) chain
NCBI Official Synonym Full Names
collagen, type X, alpha 1
NCBI Official Symbol
Col10a1
NCBI Official Synonym Symbols
Col10; Col10a-1
NCBI Protein Information
collagen alpha-1(X) chain; collagen alpha-1(X) chain; procollagen, type X, alpha 1
UniProt Protein Name
Collagen alpha-1(X) chain
Protein Family
UniProt Gene Name
Col10a1
UniProt Entry Name
COAA1_MOUSE
Uniprot Description
COL10A1: Type X collagen is a product of hypertrophic chondrocytes and has been localized to presumptive mineralization zones of hyaline cartilage. Defects in COL10A1 are the cause of Schmid type metaphyseal chondrodysplasia (SMCD). SMCD is a dominantly inherited disorder of the osseous skeleton. The cardinal features of the phenotype are mild short stature, coxa vara and a waddling gait. Radiography usually shows sclerosis of the ribs, flaring of the metaphyses, and a wide irregular growth plate, especially of the knees. A variant form of SMCD is spondylometaphyseal dysplasia Japanese type. It is characterized by spinal involvement comprising mild platyspondyly, vertebral body abnormalities, and end-plate irregularity.
Protein type: Secreted, signal peptide; Secreted
Cellular Component: proteinaceous extracellular matrix; collagen; extracellular region; cell cortex
Research Articles on COL10
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Product Notes
The Porcine COL10 col10a1 (Catalog #AAA2510606) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2510606 ELISA Kit recognizes Porcine COL10. It is sometimes possible for the material contained within the vial of "COL10, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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