Porcine Carbonic anhydrase 7 (CA7) ELISA Kit | CA7 elisa kit

Porcine Carbonic anhydrase 7 (CA7) ELISA Kit

Cat. Num. AAA7200836

• Gene Names: CA7; CAVII
• Reactivity: Porcine
• Synonyms: Carbonic anhydrase 7 (CA7); Porcine Carbonic anhydrase 7 (CA7) ELISA Kit; CA7 elisa kit

• Reactivity: Porcine

• Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 0.1 ng/mL.
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve

Related Product Information for CA7 elisa kit

Intended Uses: This CA7 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine CA7. This ELISA kit for research use only!

Principle of the Assay||CA7 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CA7 antibody and an CA7-HRP conjugate. The assay sample and buffer are incubated together with CA7-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CA7 concentration since CA7 from samples and CA7-HRP conjugate compete for the anti-CA7 antibody binding site. Since the number of sites is limited, as more sites are occupied by CA7 from the sample, fewer sites are left to bind CA7-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CA7 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CA7 elisa kit

Cardiovascular

NCBI and Uniprot Product Information

• NCBI GI #: 62240989
• NCBI GeneID: 766
• NCBI Accession #: NP_001014435.1
• NCBI GenBank Nucleotide #: NM_001014435.1
• Molecular Weight: 23,452 Da
• NCBI Official Full Name: carbonic anhydrase 7 isoform 2
• NCBI Official Synonym Full Names: carbonic anhydrase VII
• NCBI Official Symbol: CA7
• NCBI Official Synonym Symbols: CAVII
• NCBI Protein Information: carbonic anhydrase 7; CA-VII; carbonic dehydratase VII; carbonate dehydratase VII
• UniProt Protein Name: Carbonic anhydrase 7
• Protein Family: Carbonic anhydrase
• UniProt Gene Name: CA7
• UniProt Synonym Gene Names: CA-VII
• UniProt Entry Name: CAH7_HUMAN

NCBI Description

Carbonic anhydrases are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. They show extensive diversity in tissue distribution and in their subcellular localization. The cytosolic protein encoded by this gene is predominantly expressed in the salivary glands. Alternative splicing in the coding region results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]

Uniprot Description

CA7: Reversible hydration of carbon dioxide. Belongs to the alpha-carbonic anhydrase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 4.2.1.1; Lyase; Energy Metabolism - nitrogen

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: cytosol

Molecular Function: carbonate dehydratase activity; zinc ion binding

Biological Process: bicarbonate transport; positive regulation of synaptic transmission, GABAergic; positive regulation of cellular pH reduction; one-carbon compound metabolic process