Porcine Beclin-1 (BECN1) ELISA Kit | BECN1 elisa kit

Pig Beclin-1 (BECN1) ELISA Kit

Cat. Num. AAA2801367

• Gene Names: BECN1; ATG6
• Reactivity: Porcine
• Synonyms: Beclin-1 (BECN1); Pig Beclin-1 (BECN1) ELISA Kit; ATG6; VPS30; beclin1; ATG6 autophagy related 6 homolog; beclin 1; beclin 1 (coiled-coil; moesin-like BCL2 interacting protein); moesin-like BCL2-interacting protein); BECN1 elisa kit

• Reactivity: Porcine
• Specificity: This assay has high sensitivity and excellent specificity for detection of Pig BECN1. No significant cross-reactivity or interference between Pig BECN1 and analogues was observed.
• Sequence Length: 448

• Assay Type: Detection Method: Sandwich; Analysis Method: Quantitive
• Samples: Serum, Plasma, Other Biological Fluids
• Precision: Intra-assay Precision (Precision within an assay): Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision.; Inter-assay Precision (Precision between assays): Three samples of known concentration were tested in forty separate assays to assess inter-assay precision.; CV (%) = SD/meanX100; Intra-Assay: CV; Inter-Assay: CV
• Detection Wavelength: 450 nm
• Preparation and Storage: Store at 2-8 degree C.

Related Product Information for BECN1 elisa kit

This assay employs a two-site sandwich ELISA to quantitate BECN1 in samples. An antibody specific for BECN1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BECN1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BECN1 is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BECN1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 91208331
• NCBI GeneID: 733576
• UniProt Accession #: Q4A1L5
• Molecular Weight: 51,759 Da
• NCBI Official Full Name: Beclin-1
• NCBI Official Symbol: BECN1
• NCBI Official Synonym Symbols: ATG6
• NCBI Protein Information: beclin-1
• UniProt Protein Name: Beclin-1
• Protein Family: Beclin
• UniProt Gene Name: BECN1
• UniProt Synonym Gene Names: ATG6

Uniprot Description

Plays a central role in autophagy. Acts as core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate; different complex forms are believed to play a role in multiple membrane trafficking pathways: PI3KC3-C1 is involved in initiation of autophagosomes and PI3KC3-C2 in maturation of autophagosomes and endocytosis. Involved in regulation of degradative endocytic trafficking and required for the abcission step in cytokinesis, probably in the context of PI3KC3-C2. Essential for the formation of PI3KC3-C2 but not PI3KC3-C1 PI3K complex forms. Involved in endocytosis. May play a role in antiviral host defense ().