Typical Testing Data/Standard Curve (for reference only)
Porcine Alpha-1, 3-galactosyltransferase 2 (A3GALT2) ELISA Kit | A3GALT2 elisa kit
Porcine Alpha-1, 3-galactosyltransferase 2 (A3GALT2) ELISA Kit
Gene Names
A3GALT2; IGBS3S; A3GALT2P
Reactivity
Porcine
Synonyms
Alpha-1; 3-galactosyltransferase 2 (A3GALT2); Porcine Alpha-1; 3-galactosyltransferase 2 (A3GALT2) ELISA Kit; A3GALT2 elisa kit
Reactivity
Porcine
Specificity
This assay has high sensitivity and excellent specificity for detection of A3GALT2. No significant cross-reactivity or interference between A3GALT2 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between A3GALT2 and all the analogues, therefore, cross reaction may still exist in some cases.
Samples
Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for A3GALT2 elisa kit
Intended Uses: This A3GALT2 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine A3GALT2. This ELISA kit for research use only, not for therapeutic or test applications!
Principle of the Assay: A3GALT2 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-A3GALT2 antibody and an A3GALT2-HRP conjugate. The assay sample and buffer are incubated together with A3GALT2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the A3GALT2 concentration since A3GALT2 from samples and A3GALT2-HRP conjugate compete for the anti-A3GALT2 antibody binding site. Since the number of sites is limited, as more sites are occupied by A3GALT2 from the sample, fewer sites are left to bind A3GALT2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The A3GALT2 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: A3GALT2 ELISA kit applies the competitive enzyme immunoassay technique utilizing an anti-A3GALT2 antibody and an A3GALT2-HRP conjugate. The assay sample and buffer are incubated together with A3GALT2-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the A3GALT2 concentration since A3GALT2 from samples and A3GALT2-HRP conjugate compete for the anti-A3GALT2 antibody binding site. Since the number of sites is limited, as more sites are occupied by A3GALT2 from the sample, fewer sites are left to bind A3GALT2-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The A3GALT2 concentration in each sample is interpolated from this standard curve.
References
Silencing the porcine iGb3s gene does not affect Gal?3Gal levels or measures of anticipated pig?to?human and pig?to?primate acute rejection. Butler JR, Skill NJ, Priestman DL, Platt FM, Li P, Estrada JL, Martens GR, Ladowski JM, Tector M, Tector AJ (2016)
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
alpha 1,3-galactosyltransferase 2
NCBI Official Synonym Full Names
alpha 1,3-galactosyltransferase 2
NCBI Official Symbol
A3GALT2
NCBI Official Synonym Symbols
IGBS3S; A3GALT2P
NCBI Protein Information
alpha 1,3-galactosyltransferase 2; iGb3 synthase; isoglobotriaosylceramide synthase; alpha 1,3-galactosyltransferase 2, pseudogene
Protein Family
Research Articles on A3GALT2
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Product Notes
The Porcine A3GALT2 (Catalog #AAA7205937) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7205937 ELISA Kit recognizes Porcine A3GALT2. It is sometimes possible for the material contained within the vial of "Alpha-1, 3-galactosyltransferase 2 (A3GALT2), ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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