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Porcine Troponin I Type 3, Cardiac (TNNI3) DIY ELISA Kit | TNNI3 diy elisa kit

Porcine Troponin I Type 3, Cardiac (TNNI3) ELISA Kit DIY Materials

Gene Names
Tnni3; Tn1; cTnI
Reactivity
Porcine
Synonyms
Troponin I Type 3; Cardiac (TNNI3); Porcine Troponin I Type 3; Cardiac (TNNI3) ELISA Kit DIY Materials; cTnI; TNNC1; CMH7; C-TnI; Cardiac troponin I; TNNI3 diy elisa kit
Ordering
For Research Use Only!
Reactivity
Porcine
Specificity
The Abs in the kit have high sensitivity and excellent specificity for detection of Troponin I Type 3, Cardiac (TNNI3). No significant cross-reactivity or interference between Troponin I Type 3, Cardiac (TNNI3) and analogues was observed.
Sequence Length
211
Application Notes
Main materials for "Do It Yourself ELISA Kit"
Assay Type
Double-antibody Sandwich ELISA for Antigen Detection
Samples
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
Detection Range
31.2-2000pg/mL
Sensitivity
11.7pg/mL
Reagent Contents
Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
Product Usage
1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Other Reagents Required
ELISA/CLIA Support Pack 1. (Catalog MBS2089471)
Preparation and Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.
Product Categories/Family for TNNI3 diy elisa kit

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
24,259 Da
NCBI Official Full Name
troponin I, cardiac muscle
NCBI Official Synonym Full Names
troponin I, cardiac 3
NCBI Official Symbol
Tnni3
NCBI Official Synonym Symbols
Tn1; cTnI
NCBI Protein Information
troponin I, cardiac muscle
UniProt Protein Name
Troponin I, cardiac muscle
Protein Family
UniProt Gene Name
Tnni3

Uniprot Description

TNNI3: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Binds to actin and tropomyosin. Interacts with TRIM63. Interacts with STK4/MST1. Belongs to the troponin I family.

Protein type: Actin-binding; Motility/polarity/chemotaxis; Motor

Chromosomal Location of Human Ortholog: 7 A1|7 2.61 cM

Cellular Component: contractile fiber; cytoplasm; myofibril; sarcomere; troponin complex

Molecular Function: actin binding; calcium channel inhibitor activity; calcium-dependent protein binding; protein domain specific binding; protein kinase binding; troponin C binding; troponin T binding

Biological Process: cardiac muscle contraction; cellular calcium ion homeostasis; heart contraction; heart development; negative regulation of ATPase activity; regulation of muscle contraction; regulation of smooth muscle contraction; regulation of systemic arterial blood pressure by ischemic conditions; skeletal muscle contraction; striated muscle contraction; vasculogenesis; ventricular cardiac muscle morphogenesis

Research Articles on TNNI3

Similar Products

Product Notes

The Porcine TNNI3 tnni3 (Catalog #AAA2088597) is a DIY ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2088597 DIY ELISA Kit recognizes Porcine TNNI3. Main materials for "Do It Yourself ELISA Kit". Researchers should empirically determine the suitability of the TNNI3 tnni3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Troponin I Type 3, Cardiac (TNNI3), DIY ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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