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Porcine Insulin Like Growth Factor Binding Protein 1 (IGFBP1) DIY ELISA Kit | IGFBP1 diy elisa kit

Porcine Insulin Like Growth Factor Binding Protein 1 (IGFBP1) ELISA Kit DIY Materials

Gene Names
IGFBP1; AFBP; IBP1; PP12; IGF-BP25; hIGFBP-1
Reactivity
Porcine
Synonyms
Insulin Like Growth Factor Binding Protein 1 (IGFBP1); Porcine Insulin Like Growth Factor Binding Protein 1 (IGFBP1) ELISA Kit DIY Materials; IBP1; AFBP; IGF-BP25; PP12; Placental Protein 12; Amniotic Fluid Binding Protein; Alpha-Pregnancy-Associated Endometrial Globulin; Growth Hormone Independent-Binding Protein; IGFBP1 diy elisa kit
Ordering
For Research Use Only!
Reactivity
Porcine
Specificity
The Abs in the kit have high sensitivity and excellent specificity for detection of Insulin Like Growth Factor Binding Protein 1 (IGFBP1). No significant cross-reactivity or interference between Insulin Like Growth Factor Binding Protein 1 (IGFBP1) and analogues was observed.
Sequence Length
259
Application Notes
Main materials for "Do It Yourself ELISA Kit"
Assay Type
Double-antibody Sandwich ELISA for Antigen Detection
Samples
Serum, plasma and other biological fluids.
Detection Range
3.12-200ng/mL
Sensitivity
1.32ng/mL
Reagent Contents
Capture Antibody, Detection Antibody, Standard, Streptavidin-HRP, TMB Substrate, 96-well Plate
Product Usage
1. Coat the plates with 100muL per well of working solution of Capture Antibody.incubate overnight at 4 degree C or incubate at 37 degree C for 2 hours.
2. Aspirate and wash 1 time.
3. Block the plates with 200 muL per well of working solution of Blocking Buffer. Incubate at 37 degree C for 1.5 hours.
4. Aspirate and wash 1 time. The plates are now ready for sample detection, the protocol is the same as regular ELISA.
Other Reagents Required
ELISA/CLIA Support Pack 1. (Catalog MBS2089471)
Preparation and Storage
Antibodies, Standard and Streptavidin-HRP should be stored at -20 degree C. TMB should be stored at 4 degree C. 96-well Plate could be stored at room temperature. The contents are valid for twelve months. They are stable for one month after opening when stored at 4 degree C.
Product Categories/Family for IGFBP1 diy elisa kit

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
27,904 Da
NCBI Official Full Name
insulin-like growth factor-binding protein 1
NCBI Official Synonym Full Names
insulin like growth factor binding protein 1
NCBI Official Symbol
IGFBP1
NCBI Official Synonym Symbols
AFBP; IBP1; PP12; IGF-BP25; hIGFBP-1
NCBI Protein Information
insulin-like growth factor-binding protein 1
UniProt Protein Name
Insulin-like growth factor-binding protein 1
UniProt Gene Name
IGFBP1
UniProt Synonym Gene Names
IBP1; IBP-1; IGF-binding protein 1; IGFBP-1; PP12

NCBI Description

This gene is a member of the insulin-like growth factor binding protein (IGFBP) family and encodes a protein with an IGFBP domain and a thyroglobulin type-I domain. The protein binds both insulin-like growth factors (IGFs) I and II and circulates in the plasma. Binding of this protein prolongs the half-life of the IGFs and alters their interaction with cell surface receptors. [provided by RefSeq, Jul 2008]

Uniprot Description

IGFBP1: IGF-binding proteins prolong the half-life of the IGFs and have been shown to either inhibit or stimulate the growth promoting effects of the IGFs on cell culture. They alter the interaction of IGFs with their cell surface receptors. Promotes cell migration.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 7p12.3

Cellular Component: extracellular region

Molecular Function: insulin-like growth factor binding; insulin-like growth factor I binding; insulin-like growth factor II binding; receptor binding

Biological Process: cellular protein metabolic process; regulation of insulin-like growth factor receptor signaling pathway; signal transduction

Research Articles on IGFBP1

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Product Notes

The Porcine IGFBP1 igfbp1 (Catalog #AAA2088338) is a DIY ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA2088338 DIY ELISA Kit recognizes Porcine IGFBP1. Main materials for "Do It Yourself ELISA Kit". Researchers should empirically determine the suitability of the IGFBP1 igfbp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Insulin Like Growth Factor Binding Protein 1 (IGFBP1), DIY ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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