P450R Blocking Peptide | POR blocking peptide

P450R Antibody (Center) Blocking Peptide

• Gene Names: POR; CPR; CYPOR; P450R
• Synonyms: P450R; P450R Antibody (Center) Blocking Peptide; NADPH--cytochrome P450 reductase; CPR; POR; CYPOR; POR blocking peptide

• Specificity: The synthetic peptide sequence used to generate the antibody was selected from the Center region of human P450R. A 10 to 100 fold molar excess to antibody is recommended. Precise conditions should be optimized for a particular assay.
• Form/Format: Synthetic peptide was lyophilized with 100% acetonitrile and is supplied as a powder. Reconstitute with 0.1 ml DI water for a final concentration of 1 mg/ml.
• Sequence Length: 677

• Cellular Location: Endoplasmic reticulum membrane; Peripheral membrane protein. Note: Anchored to the ER membrane by its N- terminal hydrophobic region
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C.

Related Product Information for POR blocking peptide

This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5.

NCBI and Uniprot Product Information

• NCBI GI #: 2851393
• NCBI GeneID: 5447
• UniProt Accession #: P16435; Q16455; Q197M5; Q8N181; Q9H3M8; Q9UDT3
• Molecular Weight: 76,690 Da
• NCBI Official Full Name: NADPH--cytochrome P450 reductase
• NCBI Official Synonym Full Names: cytochrome p450 oxidoreductase
• NCBI Official Symbol: POR
• NCBI Official Synonym Symbols: CPR; CYPOR; P450R
• NCBI Protein Information: NADPH--cytochrome P450 reductase
• UniProt Protein Name: NADPH--cytochrome P450 reductase
• Protein Family: Pyruvate synthase
• UniProt Gene Name: POR
• UniProt Synonym Gene Names: CPR; P450R
• UniProt Entry Name: NCPR_HUMAN

NCBI Description

This gene encodes an endoplasmic reticulum membrane oxidoreductase with an FAD-binding domain and a flavodoxin-like domain. The protein binds two cofactors, FAD and FMN, which allow it to donate electrons directly from NADPH to all microsomal P450 enzymes. Mutations in this gene have been associated with various diseases, including apparent combined P450C17 and P450C21 deficiency, amenorrhea and disordered steroidogenesis, congenital adrenal hyperplasia and Antley-Bixler syndrome. [provided by RefSeq, Jul 2008]

Uniprot Description

POR: This enzyme is required for electron transfer from NADP to cytochrome P450 in microsomes. It can also provide electron transfer to heme oxygenase and cytochrome B5. Defects in POR are the cause of Antley-Bixler syndrome with genital anomalies and disordered steroidogenesis (ABS1). A disease characterized by the association of Antley-Bixler syndrome with steroidogenesis defects and abnormal genitalia. Antley-Bixler syndrome is characterized by craniosynostosis, radiohumeral synostosis present from the perinatal period, midface hypoplasia, choanal stenosis or atresia, femoral bowing and multiple joint contractures. Defects in POR are the cause of disordered steroidogenesis due to cytochrome P450 oxidoreductase deficiency (DISPORD). A disorder resulting in a rare variant of congenital adrenal hyperplasia, with apparent combined P450C17 and P450C21 deficiency and accumulation of steroid metabolites. Affected girls are born with ambiguous genitalia, but their circulating androgens are low and virilization does not progress. Conversely, affected boys are sometimes born undermasculinized. Boys and girls can present with bone malformations, in some cases resembling the pattern seen in patients with Antley-Bixler syndrome.

Protein type: EC 1.6.2.4; Oxidoreductase

Chromosomal Location of Human Ortholog: 7q11.2

Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle; membrane

Molecular Function: [methionine synthase] reductase activity; NADPH-hemoprotein reductase activity; protein binding

Biological Process: positive regulation of monooxygenase activity; xenobiotic metabolic process

Disease: Antley-bixler Syndrome With Genital Anomalies And Disordered Steroidogenesis; Antley-bixler Syndrome Without Genital Anomalies Or Disordered Steroidogenesis; Disordered Steroidogenesis Due To Cytochrome P450 Oxidoreductase Deficiency