Rabbit ZSCAN4 Polyclonal Antibody | anti-ZSCAN4 antibody

ZSCAN4 Antibody

Cat. Num. AAA9408873

• Gene Names: ZSCAN4; ZNF494
• Reactivity: Human, Mouse, Rat
• Applications: ELISA, Western Blot, Immunohistochemistry
• Purity: Affinity chromatography purified via peptide column
• Synonyms: ZSCAN4; Polyclonal Antibody; ZSCAN4 Antibody; Zinc finger and SCAN domain containing 4; ZNF494; FLJ35105; anti-ZSCAN4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Affinity chromatography purified via peptide column
• Form/Format: Supplied in PBS containing 0.02% sodium azide.
• Sequence Length: 433

• Applicable Applications for anti-ZSCAN4 antibody: ELISA (EIA), Western Blot (WB), Immunohistochemistry (IHC)
• Immunogen Type: Peptide
• Immunogen Description: Raised against a 18 amino acid peptide near the carboxy terminus of human ZSCAN4.
• Target Name: ZSCAN4
• Preparation and Storage: Can be stored at -20 degree C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Western Blot (WB)

(Western blot analysis of ZSCAN4 in mouse lung tissue lysate with ZSCAN4 antibody in (A) the absence and (B) presence of peptide blocking.)

Immunohistochemistry (IHC)

(Immunohistochemistry of ZSCAN4 in rat lung tissue with ZSCAN4 antibody at 5 ug/mL.)

Related Product Information for anti-ZSCAN4 antibody

Zytotic genome activation (ZGA) is the first and most critical transition event from the maternal genetic to the embryonic genetic program and the major burst of ZGA occurs only in the late 2-cell stage. ZSCAN4 encodes a SCAN domain and four zinc finger domains and is expressed in late 2-cell embryos and embryonic stem cells. Loss-of-function studies using siRNA shows delays in the progression from the 2-cell to the 4-cell stage and subsequent embryo development. ZSCAN4 plays an important role in the preimplantation development. Also, ZSCAN4 is involved in telomere maintenance and long term genomic stability in ES cells, suggesting that ZSCAN4 may be of critical importance in maintaining pluripotency in replicating stem cells.

Product Categories/Family for anti-ZSCAN4 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 22749365
• NCBI GeneID: 201516
• NCBI Accession #: NP_689890
• NCBI GenBank Nucleotide #: NM_152677.2
• Molecular Weight: 48,957 Da
• NCBI Official Full Name: zinc finger and SCAN domain-containing protein 4
• NCBI Official Synonym Full Names: zinc finger and SCAN domain containing 4
• NCBI Official Symbol: ZSCAN4
• NCBI Official Synonym Symbols: ZNF494
• NCBI Protein Information: zinc finger and SCAN domain-containing protein 4
• UniProt Protein Name: Zinc finger and SCAN domain-containing protein 4
• Protein Family: Zinc finger and SCAN domain-containing protein
• UniProt Gene Name: ZSCAN4
• UniProt Synonym Gene Names: ZNF494
• UniProt Entry Name: ZSCA4_HUMAN

NCBI Description

The ZSCAN4 gene encodes a protein involved in telomere maintenance and with a key role in the critical feature of mouse embryonic stem (ES) cells, namely, defying cellular senescence and maintaining normal karyotype for many cell divisions in culture (Zalzman et al., 2010 [PubMed 20336070]).[supplied by OMIM, May 2010]

Uniprot Description

ZSCAN4: Embryonic stem (ES) cell-specific transcription factor required to regulate ES cell pluripotency. Binds telomeres and plays a key role in genomic stability in ES cells by regulating telomere elongation. Acts as an activator of spontaneous telomere sister chromatid exchange (T-SCE) and telomere elongation in undifferentiated ES cells.

Protein type: Transcription factor; C2H2-type zinc finger protein

Chromosomal Location of Human Ortholog: 19q13.43

Cellular Component: nuclear chromosome, telomeric region

Molecular Function: DNA binding; metal ion binding; transcription factor activity

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent