Rabbit ZNF638 Polyclonal Antibody | anti-ZNF638 antibody

ZNF638 Polyclonal Antibody

Cat. Num. AAA3006039

• Gene Names: ZNF638; ZFML; NP220; Zfp638
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Synonyms: ZNF638; Polyclonal Antibody; ZNF638 Polyclonal Antibody; Zinc finger protein 638; Cutaneous T-cell lymphoma-associated antigen se33-1; CTCL-associated antigen se33-1; Nuclear protein 220; Zinc finger matrin-like protein; NP220; ZFML; anti-ZNF638 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: ZNF638 polyclonal antibody detects endogenous levels of ZNF638 protein.
• Purity/Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is >95% (by SDS-PAGE).
• Form/Format: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
• Sequence Length: 1978

• Applicable Applications for anti-ZNF638 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200
• Immunogen: Synthetic peptide, corresponding to amino acids 1308-1353 of Human ZNF638.
• Preparation and Storage: Store at 4 degree C short term.; Aliquot and store at -20 degree C long term.; Avoid freeze-thaw cycles.

Western Blot (WB)

(Western Blot (WB) analysis of ZNF638 polyclonal antibody at 1:500 dilutionLane1:HEK293T cell lysateLane2:NIH-T3 cell lysateLane3:H9C2 cell lyate)

Related Product Information for anti-ZNF638 antibody

Zinc-finger proteins contain DNA-binding domains and have a wide variety of functions, most of which encompass some form of transcriptional activation or repression. NP220 (Nuclear protein 220), also designated Zinc finger protein 638 (ZNF638) or Cutaneous T-cell lymphoma-associated antigen se33-1, is a 1978 amino acid protein that contains one matrin-type zinc finger and two RRM (RNA recognition motif) domains, suggesting a role in transcriptional regulation. NP220 binds to double-stranded DNA fragments by recognizing clusters of cytidines. NP220 interacts with FHL-2 and is also thought to be phosphorylated by ATM or ATR upon DNA damage. It exists as five isoforms as a result of alternative splicing events. Isoform five of NP220 is a tumor-associated antigen found in several cutaneous T-cell lymphoma (CTCL), and in particular in mycosis fungoides patients and in Sezary syndrome patients.

NCBI and Uniprot Product Information

• NCBI GI #: 62526045
• NCBI GeneID: 27332
• NCBI Accession #: NP_001014972.1
• NCBI GenBank Nucleotide #: NP_001014972.1
• UniProt Accession #: Q14966
• Molecular Weight: ~ 220kDa, 120kDa (Isoform 5)
• NCBI Official Full Name: zinc finger protein 638 isoform 1
• NCBI Official Synonym Full Names: zinc finger protein 638
• NCBI Official Symbol: ZNF638
• NCBI Official Synonym Symbols: ZFML; NP220; Zfp638
• NCBI Protein Information: zinc finger protein 638
• UniProt Protein Name: Zinc finger protein 638
• Protein Family: Zinc finger protein
• UniProt Gene Name: ZNF638
• UniProt Synonym Gene Names: NP220; ZFML; CTCL-associated antigen se33-1
• UniProt Entry Name: ZN638_HUMAN

NCBI Description

The protein encoded by this gene is a nucleoplasmic protein. It binds cytidine-rich sequences in double-stranded DNA. This protein has three types of domains: MH1, MH2 (repeated three times) and MH3. It is associated with packaging, transferring, or processing transcripts. Multiple alternatively spliced transcript variants have been found for this gene, but the biological validity of some variants has not been determined. [provided by RefSeq, Jul 2008]

Uniprot Description

ZNF638: Early regulator of adipogenesis that works as a transcription cofactor of CEBPs, controlling the expression of PPARG and probably of other proadipogenic genes, such as SREBF1. Binds to cytidine clusters in double-stranded DNA. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: DNA-binding; RNA-binding

Chromosomal Location of Human Ortholog: 2p13.1

Cellular Component: nucleoplasm; intracellular membrane-bound organelle; cytoplasm; nuclear speck

Molecular Function: zinc ion binding; RNA binding; double-stranded DNA binding; nucleotide binding

Biological Process: transcription, DNA-dependent; regulation of transcription, DNA-dependent; RNA splicing