Immunofluorescence (IF)
(Immunofluorescence staining of ZNF384 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human ZNF384 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.)
Rabbit anti-Human ZNF384 Polyclonal Antibody | anti-ZNF384 antibody
Anti-ZNF384 Antibody, Rabbit Polyclonal
Reactivity
Human
Applications
Immunocytochemistry, Immunofluorescence
Purity
Protein A & Antigen Affinity
Synonyms
ZNF384; Polyclonal Antibody; Anti-ZNF384 Antibody; Rabbit Polyclonal; ZNF384 Antibody; Rabbit PAb; Antigen Affinity Purified; Anti-CAGH1 Antibody; Anti-CAGH1A Antibody; Anti-CIZ Antibody; Anti-ERDA2 Antibody; Anti-NMP4 Antibody; Anti-NP Antibody; Anti-TNRC1 Antibody; anti-ZNF384 antibody
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
Rabbit IgG
Specificity
Human ZNF384
Purity/Purification
Protein A & Antigen Affinity
Form/Format
Liquid; PBS, pH7.0 with 0.03% Proclin300
Applicable Applications for anti-ZNF384 antibody
Immunocytochemistry (ICC), Immunofluorescence (IF)
Application Notes
ICC/IF: 1:100-1:500
Immunogen
E Coli-derived Human ZNF384 fragment
Conjugation
Unconjugated
Preparation
Produced in rabbits immunized with E Coli-derived Human ZNF384 fragment, and purified by antigen affinity chromatography.
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Avoid repeated freeze-thaw cycles.
Immunofluorescence (IF)
(Immunofluorescence staining of ZNF384 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human ZNF384 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.)
Immunofluorescence (IF)
(Immunofluorescence staining of ZNF384 in A431 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human ZNF384 polyclonal antibody (dilution ratio 1:200) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green). Positive staining was localized to Nucleus.)
Related Product Information for anti-ZNF384 antibody
Transcription factor ZNF384/CIZ/NMP4 was first cloned in rat as a p130Cas-binding protein and has a role in bone metabolism and spermatogenesis. Zinc-finger protein 384 (ZNF384) encodes a zinc finger protein, which acts as a transcription factor, regulating the expression of several matrix metalloproteinases and TAF15 belongs to the FET (FUS, EWS, and TAF15) family, consisting of RNA and DNA-binding proteins. ZNF384 gene rearrangements with multiple partner genes are recurrent in acute leukemia and are most often associated with a precursor B cell immunophenotype. ZNF384 promotes the proliferation of hepatocellular carcinoma (HCC) cells by directly upregulating the expression of Cyclin D1 and might serve as a prognostic predictive factor for HCC patients.
References
Janssen H, et al. (2006) Interaction partners for human znf384/ciz/nmp4--zyxin as a mediator for p130cas signaling? Exp Cell Res 312 (7): 1194-1204.He L, et al. (2019) Overexpression of zinc finger protein 384 (znf 384), a poor prognostic predictor, promotes cell growth by upregulating the expression of cyclin d1 in hepatocellular carcinoma. Cell Death Dis 10 (6): 444.Georgakopoulos N, et al. (2018) An adult patient with early pre-b acute lymphoblastic leukemia with t(12;17) (p13;q21)/znf384-taf15. In Vivo 32 (5): 1241-1245.
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Product Notes
The ZNF384 (Catalog #AAA8110309) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-ZNF384 Antibody, Rabbit Polyclonal reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's ZNF384 can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunofluorescence (IF). ICC/IF: 1:100-1:500. Researchers should empirically determine the suitability of the ZNF384 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "ZNF384, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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